›› 1991, Vol. 0 ›› Issue (3): 10-13.

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Determination of Human Seminal Stains by Sandwich Indirect ELISA

GOU QING HOU YIPING MAO YONGQIOU WU MEIYUNDEPARTMENT OF FORENSIC SEROLOGYWEST CHINA UNIVERSITY OF MEDICAL SCIENCES   

  • Online:1991-06-25 Published:1991-06-28

Abstract: For the purpose of confirming the human semen stains, a sandwich indirect ELISA test was established using the rabbite anti-human p30 serum and goat antihuman p30 serum. This method is sensitive enough to detect 200ng/ml p30. A blind test to examine 126 biological stains which have boon kept at room temperature for one year was carried out using this method.The test results showed 100% accuracy.There were not any false-positive and false-negative results.

Key words: Enzyme-linked immunosorbent assay Human prostatespecific antigen p30 Conclusive test of semen stains The Project Supported by National Science Foundation of China