Abstract: A reverse - phase High - performance liquid chromatography for the detection of aconitine concentration in biological samples of rabbits was used. This method showed good linearity,precision and accuracy over range of 0. 05 5ug/ml. After ig Aconitum brachypodum Diels 1. 5g/kg, it was showed that aconitine was a two-conmpartnlent open model drug in rabbits.Ka,A and B were 1. 1629± 0. 4053, 0. 6045 ±0. 2574, 1. 1607± 0. 3781 ug/ml separately. The influence of ethanol on toxicokinetics of aconitine in blood samples of rabbits indicated that absorption rate constant and distribution rate constant in ethanol group were greater than those in control group (p< 0. 01 ). However, there were no significant differences be tween elimination rate constant of the two groups. Ka, A and B are 2. 4026 ± 0. 4053,1. 2051 ± 0. 5328, 1. 2037 ± 0.4095 ug/ml separately. Our data suggested that ethanol causes the lucrease of toxicity of Aconitum brachypodum Diels.

Key words: aconitine, ethanol, HPLC, interaction, toxicokinetics