›› 1996, Vol. 0 ›› Issue (1): 0-0.

• 论文 •    

A Simple Method for Extracting DNA from Polyacrylamide Gels and Amplifying DNA Directly

NI XINGQUN ; GUO JINGYUAN (DEPARTMENT OF FORENSIC SEROLOGY, SUN YAL-AEN UNIVERSITY OF MEDICAL SCIENCES; GUANGDONG 510089)   

  • Online:1996-02-25 Published:1996-02-28

Abstract: In PCR buffer, the DNA was recoveted from polyacrylamide gels by electroelution. The recovery efficiency was 0. 89± 0. 05. Added the PCR reagents,the DNA was directly amplified and the future analysis was performed. This method is easy, quick, inexpensive and highly efficient.

Key words: extracting DNA, PCR, acrylamidegels