DNA Profiling from Degrative DNA by Polymerase Chain Reaction(PCR):Recovered the Larger DNA Fragments by Electrophoretic Elution

Abstract

Abstract: The cause profiling unsuccessfully the degradative DNA by the polymerase chain reaction(PCR) was analysed.A new view was advanced.There were a lot of small DNA fragments in the degradative DNA It blocked the primer annealing and the primer extension.In this article the degradative DNA was separated by agarose gel electrophoresis.The small DNA fragments were cut off The larger DNA fragments were recovered froom the gel by electrophoretic elution and were successfully typed by PCR.This method was easy to operate.

Key words: degradativc DNA, electrophoretic elution, polymerase chain reaction

NI XINGQUN;GUO JINYUAN;CHEN LIXIAN AND GUO YUNRONG(DEPARTMENT OF FORENSIC SEROLOGY;SUN YAT-SEN UNIVERSITY OF MEDICAL SCIENCES GUANGZHOU 00). DNA Profiling from Degrative DNA by Polymerase Chain Reaction(PCR):Recovered the Larger DNA Fragments by Electrophoretic Elution[J]. , 1996, 0(4): 0-0.

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DNA Profiling from Degrative DNA by Polymerase Chain Reaction(PCR):Recovered the Larger DNA Fragments by Electrophoretic Elution

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