Study on the HLA-DRB1 Polymorphism Determined by High-resolution PCR-SBT in a Population of Beijing Region

Abstract

Abstract: Objective To investigate the polymorphism of HLA-DRB1 gene in a population of Beijing region and research on the application feasibility of HLA-DRB1 by PCR-SBT method. Methods Polymerase chain reaction sequence-based typing (PCR-SBT) method was used to determine HLA-DRB1 genotypes of 494 unrelated healthy people of Beijing region. Results A total of 233 different HLA-DRB1 genetypes and 102 alleles were detected. The observed heterozygosity (Ho), expected heterozygosity (He), polymorphism information content (PIC), probability of matching (Pm); discrimination power (DP) and probability of paternity exclusion (PPE) of HLA-DRB1 in the samples were calculated and their values were 0.921 0,0.934 2, 0.933 3, 0.010 4, 0.989 6,and 0.777 6 respectively. Conclusion HLA-DRB1 typing by PCR-SBT is very useful in forensic science.

Key words: HLA-DRB1, PCR-SBT, DNA typing, gene frequency, forensic science

DENG YA-JUN;YANG GUANG;WU DONG-YING;ET AL. (.THE NATIONAL LABORATORY OF THE MINISTRY OF HEALTH FOR FORENSIC SCIENCES;XI′AN JIAOTONG UNIVERSITY;XI′AN 00;CHINA;.BEIJING GENOMICS INSTITUTE;CHINESE ACADEMY OF SCIENCES;BEIJING 000;CHI. Study on the HLA-DRB1 Polymorphism Determined by High-resolution PCR-SBT in a Population of Beijing Region[J]. , 2005, 0(4): 280-282,285.

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Study on the HLA-DRB1 Polymorphism Determined by High-resolution PCR-SBT in a Population of Beijing Region

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