Detecting the Haplotype of Three Y-STR Loci by Fluorescent Multiplex Amplification

Abstract

Abstract: Objective To establish a fluorescent multiplex PCR system for typing Y-STR loci Y-GATA-A7.1, DYS456 and DYS443, and investigate their haplotype frequencies in Chinese Han population. Methods 203 unrelated males of Han population living in Zhengzhou were typed by fluorescent multiplex amplification system and ABI 3100 genetic analyzer. Results In Zhengzhou Han population, 5,6 and 6 different alleles were observed for Y-GATA-A7.1, DYS456 and DYS443 loci, and their gene diversity (GD) were 0.669 2, 0.583 9 and 0.705 3 respectively. A total of 44 different haplotypes formed by these three loci was identified and the haplotype diversity (HD) reached 0.952 3. Conclusion The fluorescent multiplex system for these three Y-STR loci will be very powerful for forensic individual identification and paternity testing in Chinese Han population.

Key words: Y-STRs, multiplex amplification, haplotype, Y-GATA-A7.1, DYS456, DYS443

LIN XIAO-YAN;WANG LEI;YUAN BAO-LONG;FENG CHANG-JUN;HUANG DAI-XIN (.FACULTY OF FORENSIC MEDICINE;TONGJI MEDICAL COLLEGE;HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY;WUHAN 000;CHINA;.ZHENGZHOU PUBLIC SECURITY BUREAU;ZHENGZHOU 000;CHINA. Detecting the Haplotype of Three Y-STR Loci by Fluorescent Multiplex Amplification[J]. , 2006, 0(2): 122-124.

[1]	Zheng TAN, Guan-ju MA, Li-hong FU, Xiao-jing ZHANG, Qian WANG, Guang-ping FU, Qing-qing DU, Shu-jin LI. Identification Strategy of Biological Half Sibling Relationship [J]. Journal of Forensic Medicine, 2023, 39(3): 262-270.
[2]	Xuan TANG, Dan WEN, Chu-dong WANG, Ru-yi XU, Hong-tao JIA, Jie-nan LI, Bai-yi-la ZHALAGA. Application of Microhaplotypes in Sibling Kinship Testing [J]. Journal of Forensic Medicine, 2023, 39(3): 288-295.
[3]	Qing-guo WANG, Lei ZHAO, Tang-song LI, Wang FU, He-xin XIE, Yuan MA, Wen-ping SUN, Jun-ping HAN. Optimization and Developmental Validation of 38-plex InDel Panel for Ancestry Inference [J]. Journal of Forensic Medicine, 2022, 38(5): 611-617.
[4]	Shi-han XI, Yi-ling QU, Ruo-cheng XIA, Lei XIONG, Si-yu CHAI, Chun-lan TONG, Rui-yang TAO, Cheng-tao LI. Establishment of Multiplex Amplification System of STR Loci in Felis Catus and Its Forensic Application [J]. Journal of Forensic Medicine, 2022, 38(2): 231-238.
[5]	XIAO Yue, DENG Pan, CHANG Kai-chuang，et al.. Network Analysis of Y-STR in Six Ethnic Populations in Guangxi and Its Forensic Significances [J]. 法医学杂志, 2019, 35(3): 314-318.
[6]	LI Min, HUANG Lei, WANG Xin-jie，et al. Assessment on Application of 24 Y-STR Loci in Forensic Science [J]. , 2018, 34(3): 236-241.
[7]	LIU HONG, LI YUE, LIU CHANG-HUI, ET AL.. Development of a Fluorescence Multiplex Amplification System with 24 Y-STR Loci [J]. , 2016, 32(3): 180-183.
[8]	LUO JI-HUAI, SUN HONG-BING, YANG XIN, ET AL.. Genetic Polymorphism of 24 Y-STR Loci in Dongxiang Ethnic Minority Male Residents in Gansu Province of China [J]. , 2016, 32(3): 219-221.
[9]	NIE YAN-CHAI, ZHANG CHEN, LIU YA-NAN, ET AL.. AS-PCR Assay for 20 mtDNA SNP Typing and Haplotype Frequency [J]. , 2014, 30(2): 96-100.
[10]	YE YI;LUO HAI-BO;HOU YI-PING (DEPARTMENT OF FORENSIC BIOLOGY;WEST CHINA SCHOOL OF PRECLINICAL AND FORENSIC MEDICINE;SICHUAN UNIVERSITY;CHENGDU 00;CHINA). Analysis and Application of Haplotype in Forensic Medicine [J]. , 2009, 0(2): 133-137.
[11]	WANG RUI-HENG;LIU LI-MIN;ZHAO JIN-LING;SUN XUE-KE;SUN LIN-LIN;ZHOU GANG (. SCHOOL OF FORENSIC MEDICINE;CHINA MEDICAL UNIVERSITY;SHENYANG 000;CHINA;. SCHOOL OF LAW;LIAONING NORMAL UNIVERSITY;DALIAN 0;CHINA). A New Method for SNP Typing Based on Allele Specific PCR [J]. , 2008, 0(3): 189-193.
[12]	YANG FAN;MEI SHAN-ZONG;LI YONG-HONG;FENG YAN;YU WEI-DONG;ZHANG YUE (.DEPARTMENT OF FORENSIC MEDICINE;WANNAN MEDICAL UNIVERSITY;WUHU 00;CHINA;.CENTER OF FORENSIC IDENTIFICATION;NANJING PUBLIC SECURITY BUREAU;NANJING 0000;CHINA). STR Genotyping from Trace Epithelial Cells on Fountain Pen [J]. , 2008, 0(1): 34-37.
[13]	YU BING;TUO YA;GUI HONG-SHENG;LI SHENG-BIN (KEY LABORATORY OF FORENSIC SCIENCES;NATIONAL MINISTRY OF HEALTH;KEY LABORATORY OF MINISTRY OF EDUCATION FOR ENVIRONMENT AND GENES RELATED TO DISEASES;XI’AN JIAOTONG UNIVERSITY;XI’AN;00 CHINA). Validation the Haplotype Polymorphisms of the DXS7424-DXS101 on X-chromosome [J]. , 2007, 0(5): 342-344.
[14]	ZHAI XIAN-DUN;HUANG DAI-XIN;YIN HUI;YANG QIN-EN(.DEPARTMENT OF FORENSIC MEDICINE;TONGJI MEDICAL COLLEGE;HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY;WUHAN 000;CHINA;.DEPARTMENT OF FORENSIC MEDICINE;HENAN UNIVERSITY OF SCIENCE AND TECHNOLOGY;LU. Haplotype and Heteroplasmy in the HVⅡ Region of mtDNA in Human Blood Detected by PCR-DGGE [J]. , 2007, 0(1): 30-32.
[15]	ZHANG XIAO-HONG;WU WEI-WEI;TANG JIAN-XIN;QIAN GUANG-LI;ZHANG XIAO-MING(.PANYU DIVISION OF GUANGZHOU PUBLIC SECURITY BUREAU;GUANGZHOU 00;CHINA;.DEPARTMENT OF CRIMINAL INVESTIGATION;ZHEJIANG PROVINCIAL PUBLIC SECURITY BUREAU;HANGZHOU 000;CHIN. Polymorphisms of Eleven Y-chromosome STR loci and Forensic Application in Yunnan Han Population [J]. , 2006, 0(3): 210-212+.

Detecting the Haplotype of Three Y-STR Loci by Fluorescent Multiplex Amplification

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics