DNA Extraction from Formalin Fixed and Paraffin Embedded Tissues

Abstract

Abstract: Owing to the DNA degradation induced by formalin and the obstruction of paraffin to DNA extraction, it is difficult to recover high-quality DNA from Formalin fixed and paraffin embedded tissue(FFPET).In recent years, a lot of researches indicate that the DNA extraction from FFPET can be developed by improving the pretreatment,optimizing the digestion condition of proteinase, simplizing the procedures of the DNA extraction, purifying the extracted DNA and so on, which may pave a way for popularizing FFPET in DNA analysis.

Key words: formalin fixed, paraffin embedded tissue, DNA extraction

TAN ZHUO-YI;DING MEI (SCHOOL OF FORENSIC MEDICINE;CHINA MEDICAL UNIVERSITY;SHENYANG 000;CHINA). DNA Extraction from Formalin Fixed and Paraffin Embedded Tissues[J]. , 2006, 0(6): 455-458.

[1]	Jie CAI, Bo WANG, Sun-lin HU, Yi-hong QU, Tao SONG, Jian-hua CHEN, Jian-qiang DENG. Improved Glass Bead-Vortex Oscillation Method for DNA Extraction from Diatom [J]. Journal of Forensic Medicine, 2022, 38(1): 119-126.
[2]	XIN Yang. Research Progress on Touch DNA on Cartridge Cases in Forensic Field [J]. Journal of Forensic Medicine, 2021, 37(4): 555-560.
[3]	LI Shu-xia, SHEN Jin-jian, CAO Pei-jun, et al.. Application of Automatic Nucleic Acid Extractor Combined with Vacuum Concentrator in Forensic Science [J]. Journal of Forensic Medicine, 2021, 37(2): 220-224.
[4]	CAI Wei , YU Lei. Application of Two Kinds of Automatic Nucleic Acid Purifiers [J]. Journal of Forensic Medicine, 2020, 36(6): 797-800.
[5]	BAI Xiao-gang, JIAN Hui, WANG Hui, et al.. Comparison of Single Piece of Dandruff DNA Extraction under Microscope and EZ-tape Method [J]. 法医学杂志, 2018, 34(4): 401-404.
[6]	GAO LIN-LIN, XU NIAN-LAI, XIE WEI, ET AL.. DNA Extraction from Bones and Teeth Using AutoMate ExpressTM Forensic DNA Extraction System [J]. , 2013, 29(2): 127-129.
[7]	BA HUA-JIE;LIU BING-QUAN;MA JUN;ZHU AI-HUA;LIN ZI-QING (.CHANGZHOU PUBLIC SECURITY BUREAU;CHANGZHOU 00;CHINA;.CHINA CRIMINAL POLICE COLLEGE;SHENYANG 00;CHINA). Comparative research of the influence factors of DNA extraction of bloodstain on the filter paper with Chelex-100 method [J]. , 2007, 0(5): 347-348.
[8]	XU QING-WEN;WU DAN;HU WEI (SHANGHAI INSTITUTE OF CRIMINAL SCIENCE AND TECHNOLOGY;SHANGHAI 000;CHINA). Comparative Study of Two Methods of DNA Extraction from Different Colour Costal Cartilage in STR Genotyping [J]. , 2006, 0(6): 436-437.
[9]	ZHANG JIE;XUE QING-JIE;TANG GUANG-FENG;PENG-LEI (.CRIMINAL POLICE DEPARTMENT OF TAIAN CITY PUBLIC SECURITY BUREAU;TAIAN 000;CHINA;.JINING MEDICINAL COLLEGE;JINING 00;CHINA). DNA Extraction from Old Bones [J]. , 2006, 0(1): 72-73.
[10]	LIU CHAO;WANG SUIBAO;LIANG SHANGYOU (GUANGZHOU CRIMINAL SCIENCE AND TECHNOLOGY INSTITUTE;GUANGZHOU000). DNA extraction and and determination of human skeletal remanis [J]. , 1998, 0(4): 195-196+.

DNA Extraction from Formalin Fixed and Paraffin Embedded Tissues

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