Haplotype and Heteroplasmy in the HVⅡ Region of mtDNA in Human Blood Detected by PCR-DGGE

Abstract

Abstract: Objective To establish a simple and effective technique for detecting haplotype and heteroplasmy of mtDNA,and investigate their frequencies in Chinese Han population.Methods The fragments from 29～290 nt of mtDNA HVⅡ from peripheral leukocytes of 200 unrelated Wuhan Han individuals were analyzed by using PCR-DGGE technique.Results Seventeen haplotypes were found in the range of 29～290 nt,and the haplotype diversity(HD) was 0.884 4.The heterogeneity was observed from 4 individuals,and its frequency was 2%.Conclusion PCR-DGGE is a simple,sensitive and effective technique in analyzing polymorphism and heteroplasmy of mtDNA,and can be used in forensic practice.

Key words: mtDNA, denaturing gradient gel electrophoresis, Haplotype, Heteroplasmy

ZHAI XIAN-DUN;HUANG DAI-XIN;YIN HUI;YANG QIN-EN(.DEPARTMENT OF FORENSIC MEDICINE;TONGJI MEDICAL COLLEGE;HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY;WUHAN 000;CHINA;.DEPARTMENT OF FORENSIC MEDICINE;HENAN UNIVERSITY OF SCIENCE AND TECHNOLOGY;LU. Haplotype and Heteroplasmy in the HVⅡ Region of mtDNA in Human Blood Detected by PCR-DGGE[J]. , 2007, 0(1): 30-32.

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Haplotype and Heteroplasmy in the HVⅡ Region of mtDNA in Human Blood Detected by PCR-DGGE

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