Abstract: In forensic toxicology analysis, various types of biological samples have their own special characteristics and scope of applications. In this article, the physiological structure of nails, methods for collecting and pre-processing samples, and for analyzing some poisons and drugs in the nails are reviewed with details. This paper introduces the influence factors of drug abuse of the nails. The prospects of its further applications are concluded based on the research results. Nails， as an unconventional bio-sample without general application, show great potential and advantages in forensic toxicology.

Key words: forensic toxicology, substance-related disorders, review[publication type], nails