Abstract: Objective To investigate the genetic data of 16 X-STR loci in Henan Han population and to assess the application value in forensic science. Methods The DNA of 326 unrelated individuals in Henan Han population were amplified using GoldeneyeTM DNA identification system 17X kit, and the PCR products were analyzed by electrophoresis through 3130xl genetic analyzer. The fragment sizes of alleles were analyzed subsequently by GeneMapper?誖ID-X. Allele frequencies and population genetics parameters of 16 X-STR loci were analyzed statistically and compared with the available data of other Han populations from different regions. Results Among the 16 X-STR loci, DXS6800 were found to be moderately polymorphic and the other 15 X-STR loci were highly polymorphic. The cumulative discrimination power in females and males were 0.999 999 999 999 992 and 0.999 999 996 577 712, respectively. The combined power of exclusion in trios and in duos were 0.999 999 971 and 0.999 992 574, respectively. Conclusion The 16 X-STR loci meet the application requires of forensic genetics, especially for testing the special paternity cases.

Key words:  forensic genetics, polymorphism, genetic, X-STR, Henan, Han nationality