Journal of Forensic Medicine

›› 2018, Vol. 34 ›› Issue (2): 126-131,137.DOI: 10.3969/j.issn.1004-5619.2018.02.004

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Establishment of 43-plex SNP Typing System and Its Forensic Application

LI Ya-nan1,2, LI Min2,3, JIANG Lei2, LUAN Xiao-hui4, LIANG Na4, XU Qian-nan2,3, ZHANG Jia-shuo2,5, TANG Ming-chi6, BIAN Ying-nan2, CHEN Li-qin1   

  1. 1. School of Basic Medicine, Inner Mongolia Medical University, Hohhot 010030, China; 2. Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Academy of Forensic Science, Shanghai 200063, China; 3. Department of Forensic Medicine, Wenzhou Medical University, Wenzhou 325035,
    China; 4. Agena Bioscience （Shanghai）Co., Ltd., Shanghai 200032, China; 5. Department of Forensic Medicine, Medical College of Soochow University, Suzhou 215123, China; 6. Criminal Technology Branch, Daoli Branch of Harbin Public Security Bureau, Harbin 150070, China
  • Online:2018-04-25 Published:2018-04-28
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Abstract:

Objective To evaluate the application of 43 -plex SNP typing system in forensic science. Methods The typing of 43 SNP loci in 123 unrelated Han individuals from East China was detected by MALDI-TOF-MS. The application value of 43-plex SNP typing system was assessed according to the forensic parameters of population genetics. Results All the 43 SNP loci of 123 individuals showed no significant departure from Hardy-Weinberg equilibrium （P>0.05）. Excepted rs1355366, rs2270529, rs10776839 and rs938283, there were 39 SNP loci had minor allele frequencies （MAF）, which were greater than 0.25. Among the 25 loci MAFs, 24 ranged from 0.4 to 0.5, while 3 were close to 0.4. The DP, CDP, PIC, Ho, PEtrio and PEduo of the 43 SNP loci were 0.290 1-0.654 4, 1-9.8×10-11, 0.170 8-0.500 0, 0.155 7-0.593 5, 0.085 4-0.250 0 and 0.014 6-0.125 0, respectively. The CPEtrio and CPEduo were 0.999 986 and 0.992 436 1,
respectively. Conclusion The 43-plex SNP typing system in present study shows a high polymorphism, which can be an effective supplement and verification for traditional STR genetic markers. It also can be used with other commercial kits for the forensic paternity testing and individual identification.

 

Key words: forensic genetics, polymorphism, genetic, matrix -assisted laser desorption -ionization, mass spectrometry, single nucleotide polymorphism, Han nationality, Eastern China

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