Journal of Forensic Medicine ›› 2023, Vol. 39 ›› Issue (2): 121-128.DOI: 10.12116/j.issn.1004-5619.2021.310802
• Original Article • Previous Articles Next Articles
Wu LONG1(), Peng-fei QU2(), Lin MA3, Rui WANG1, Yan-mei XI3, Yu-hua LI1, Sheng-jie NIE1, Ting DUAN1, Jin-liang DU1, Xue TANG3, Jing-feng ZHAO4, Pu-ping LEI1(), Yue-bing WANG3()
Received:
2021-08-12
Online:
2023-06-06
Published:
2023-04-25
Contact:
Pu-ping LEI,Yue-bing WANG
CLC Number:
Wu LONG, Peng-fei QU, Lin MA, Rui WANG, Yan-mei XI, Yu-hua LI, Sheng-jie NIE, Ting DUAN, Jin-liang DU, Xue TANG, Jing-feng ZHAO, Pu-ping LEI, Yue-bing WANG. Cytotoxicity of 4 Wild Mushrooms in a Case of Yunnan Sudden Unexplained Death[J]. Journal of Forensic Medicine, 2023, 39(2): 121-128.
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URL: http://www.fyxzz.cn/EN/10.12116/j.issn.1004-5619.2021.310802
质量浓度/(mg·mL-1) | 450 nm处吸光度 | |||
---|---|---|---|---|
美味牛肝菌 | 变绿红菇 | 隐花青鹅膏 | 粉黄黄肉牛肝菌 | |
01) | 1.48±0.05 | 1.43±0.09 | 1.32±0.02 | 1.47±0.04 |
0.001 | 1.49±0.03 | 1.41±0.02 | 1.35±0.02 | 1.40±0.02 |
0.005 | 1.48±0.02 | 1.41±0.02 | 1.34±0.03 | 1.42±0.08 |
0.01 | 1.44±0.04 | 1.45±0.03 | 1.34±0.03 | 1.44±0.01 |
0.05 | 1.38±0.09 | 1.38±0.03 | 1.36±0.02 | 1.42±0.02 |
0.1 | 1.43±0.06 | 1.43±0.07 | 1.21±0.022) | 1.45±0.07 |
0.5 | 1.44±0.06 | 1.39±0.05 | 0.58±0.032) | 1.43±0.06 |
1.0 | 1.40±0.09 | 1.42±0.08 | 0.33±0.032) | 1.44±0.06 |
Tab. 1 Cytotoxicity of raw extracts of 4 wild mushrooms to HEK293 cells detected by CCK-8 [n=3,xˉ±s,L/(g·cm)]
质量浓度/(mg·mL-1) | 450 nm处吸光度 | |||
---|---|---|---|---|
美味牛肝菌 | 变绿红菇 | 隐花青鹅膏 | 粉黄黄肉牛肝菌 | |
01) | 1.48±0.05 | 1.43±0.09 | 1.32±0.02 | 1.47±0.04 |
0.001 | 1.49±0.03 | 1.41±0.02 | 1.35±0.02 | 1.40±0.02 |
0.005 | 1.48±0.02 | 1.41±0.02 | 1.34±0.03 | 1.42±0.08 |
0.01 | 1.44±0.04 | 1.45±0.03 | 1.34±0.03 | 1.44±0.01 |
0.05 | 1.38±0.09 | 1.38±0.03 | 1.36±0.02 | 1.42±0.02 |
0.1 | 1.43±0.06 | 1.43±0.07 | 1.21±0.022) | 1.45±0.07 |
0.5 | 1.44±0.06 | 1.39±0.05 | 0.58±0.032) | 1.43±0.06 |
1.0 | 1.40±0.09 | 1.42±0.08 | 0.33±0.032) | 1.44±0.06 |
质量浓度/ (mg·mL-1) | 450 nm处吸光度 | ||
---|---|---|---|
生品浸膏 | 熬煮浸膏 | 熬煮后酶解浸膏 | |
01) | 1.45±0.05 | 1.50±0.01 | 1.50±0.01 |
0.1 | 1.25±0.062) | 1.47±0.01 | 1.54±0.04 |
0.2 | 1.13±0.032) | 1.39±0.01 | 1.56±0.01 |
0.3 | 1.01±0.032) | 1.36±0.01 | 1.50±0.01 |
0.4 | 0.62±0.032) | 1.11±0.032) | 1.49±0.02 |
0.5 | 0.45±0.042) | 0.76±0.022) | 1.45±0.02 |
0.6 | 0.44±0.012) | 0.55±0.052) | 1.41±0.03 |
0.7 | 0.35±0.012) | 0.50±0.012) | 1.17±0.062) |
0.8 | 0.31±0.012) | 0.45±0.022) | 1.14±0.052) |
0.9 | 0.30±0.012) | 0.34±0.012) | 1.03±0.072) |
1.0 | 0.30±0.012) | 0.32±0.022) | 0.93±0.062) |
Tab. 2 Cytotoxicity of 3 kinds of Amanita manginiana extracts to HEK293 cells detected by CCK-8
质量浓度/ (mg·mL-1) | 450 nm处吸光度 | ||
---|---|---|---|
生品浸膏 | 熬煮浸膏 | 熬煮后酶解浸膏 | |
01) | 1.45±0.05 | 1.50±0.01 | 1.50±0.01 |
0.1 | 1.25±0.062) | 1.47±0.01 | 1.54±0.04 |
0.2 | 1.13±0.032) | 1.39±0.01 | 1.56±0.01 |
0.3 | 1.01±0.032) | 1.36±0.01 | 1.50±0.01 |
0.4 | 0.62±0.032) | 1.11±0.032) | 1.49±0.02 |
0.5 | 0.45±0.042) | 0.76±0.022) | 1.45±0.02 |
0.6 | 0.44±0.012) | 0.55±0.052) | 1.41±0.03 |
0.7 | 0.35±0.012) | 0.50±0.012) | 1.17±0.062) |
0.8 | 0.31±0.012) | 0.45±0.022) | 1.14±0.052) |
0.9 | 0.30±0.012) | 0.34±0.012) | 1.03±0.072) |
1.0 | 0.30±0.012) | 0.32±0.022) | 0.93±0.062) |
质量浓度/(mg·mL-1) | 490 nm处吸光度 | ||
---|---|---|---|
生品浸膏 | 熬煮浸膏 | 熬煮后酶解浸膏 | |
01) | 0.20±0.01 | 0.21±0.01 | 0.21±0.01 |
0.1 | 0.23±0.01 | 0.21±0.01 | 0.20±0.01 |
0.2 | 0.29±0.012) | 0.22±0.01 | 0.20±0.00 |
0.3 | 0.30±0.012) | 0.25±0.01 | 0.23±0.01 |
0.4 | 0.33±0.012) | 0.27±0.00 | 0.21±0.01 |
0.5 | 0.42±0.032) | 0.28±0.01 | 0.22±0.00 |
0.6 | 0.78±0.032) | 0.43±0.012) | 0.23±0.01 |
0.7 | 0.88±0.032) | 0.69±0.012) | 0.29±0.012) |
0.8 | 0.92±0.012) | 0.89±0.012) | 0.51±0.022) |
0.9 | 1.00±0.062) | 1.00±0.012) | 0.73±0.012) |
1.0 | 1.00±0.052) | 1.04±0.102) | 0.87±0.022) |
Tab. 3 Cytotoxicity of 3 kinds of Amanita manginiana extracts to HEK293 cells detected by LDH Assay Kit
质量浓度/(mg·mL-1) | 490 nm处吸光度 | ||
---|---|---|---|
生品浸膏 | 熬煮浸膏 | 熬煮后酶解浸膏 | |
01) | 0.20±0.01 | 0.21±0.01 | 0.21±0.01 |
0.1 | 0.23±0.01 | 0.21±0.01 | 0.20±0.01 |
0.2 | 0.29±0.012) | 0.22±0.01 | 0.20±0.00 |
0.3 | 0.30±0.012) | 0.25±0.01 | 0.23±0.01 |
0.4 | 0.33±0.012) | 0.27±0.00 | 0.21±0.01 |
0.5 | 0.42±0.032) | 0.28±0.01 | 0.22±0.00 |
0.6 | 0.78±0.032) | 0.43±0.012) | 0.23±0.01 |
0.7 | 0.88±0.032) | 0.69±0.012) | 0.29±0.012) |
0.8 | 0.92±0.012) | 0.89±0.012) | 0.51±0.022) |
0.9 | 1.00±0.062) | 1.00±0.012) | 0.73±0.012) |
1.0 | 1.00±0.052) | 1.04±0.102) | 0.87±0.022) |
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