Research Progress on the Application of MALDI-MSI in Hair Analysis

doi:10.12116/j.issn.1004-5619.2024.340405

Abstract

Abstract:

Mass spectrometry imaging （MSI） is an emerging high-tech “photography” method combining mass spectrometry and image visualization technologies, which can simultaneously monitor the spatial distribution of multiple molecules in biological samples. In recent years, the characteristics of this technology have been used to visually study the distribution of drugs in hair, among which matrix-assisted laser desorption ionization-mass spectrometry imaging（MALDI-MSI） technology has been most widely applied in hair analysis. Based on the principle of MALDI-MSI technology, this paper discusses the key technical elements of MALDI-MSI technology applied to hair analysis and reviews the research achievements of MSI in hair analysis. It concludes that improving the ionization efficiency, resolution, sensitivity and stability of MALDI-MSI technology remains the future exploration direction, and simplifying data analysis and establishing databases will help promote MALDI-MSI as a routine analytical technique for hair analysis.

Key words: forensic medicine, toxicological analysis, matrix-assisted laser desorption ionization-mass spectrometry imaging （MALDI-MSI）, hair, key technology, data processing, visualization, review

CLC Number:

Jiao-jiao JI, Xin WANG, Jia-man LIN, Duo-qi XU, Hui YAN, Min SHEN. Research Progress on the Application of MALDI-MSI in Hair Analysis[J]. Journal of Forensic Medicine, 2024, 40(6): 542-549.

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URL: http://www.fyxzz.cn/EN/10.12116/j.issn.1004-5619.2024.340405

http://www.fyxzz.cn/EN/Y2024/V40/I6/542

Figures/Tables 2

References 53

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序号	基质	喷涂方式	分析物	文献
1	CHCA	自动喷雾器（ImagePrep）	甲氧那明	[31]
2	CHCA	薄层色谱法	可卡因	[27]
3	CHCA	自动喷雾器（SunCollect）	四氢大麻酚、大麻二酚	[36]
4	CHCA	自制尖头点涂	可卡因、苯甲酰爱康宁	[44]
5	CHCA	薄层色谱法	替利定、去甲替利定	[38]
6	CHCA	自动喷雾器（ImagePrep）	可卡因	[34]
7	CHCA	薄层色谱法	可卡因、美沙酮	[45]
8	CHCA	薄层色谱法	唑吡坦	[46]
9	CHCA	自制尖头点涂	氯胺酮	[47]
10	七叶苷	自制尖头点涂	奥氮平	[15]
11	伞形酮	自制尖头点涂	甲基苯丙胺	[48]

序号	基质	喷涂方式	分析物	文献
1	CHCA	自动喷雾器（ImagePrep）	甲氧那明	[31]
2	CHCA	薄层色谱法	可卡因	[27]
3	CHCA	自动喷雾器（SunCollect）	四氢大麻酚、大麻二酚	[36]
4	CHCA	自制尖头点涂	可卡因、苯甲酰爱康宁	[44]
5	CHCA	薄层色谱法	替利定、去甲替利定	[38]
6	CHCA	自动喷雾器（ImagePrep）	可卡因	[34]
7	CHCA	薄层色谱法	可卡因、美沙酮	[45]
8	CHCA	薄层色谱法	唑吡坦	[46]
9	CHCA	自制尖头点涂	氯胺酮	[47]
10	七叶苷	自制尖头点涂	奥氮平	[15]
11	伞形酮	自制尖头点涂	甲基苯丙胺	[48]

序号	MALDI仪器	激光波长/nm	激光斑点大小/μm	激光频率/Hz	分辨率/μm	文献
1	ultrafleXtreme MALDI-TOF/TOF质谱仪	355	直径约90	1 000	100	[31]
2	MALDI FlashQuant Workstation	355	100×200	1 000	100或200	[38-39，45]
3	MALDI SYNAPT HDMS	355	100×150	200	150×50	[34]
4	MALDI SolariX FTICR	355	直径30	1 000	500	[25]
5	MALDI SolariX FTICR	355	直径100	25	500	[48]
6	MALDI-QqQ_LIT	355	200×100	1 000	1 000	[27]
7	iMScope TRIO MALDI-IT-TOF	355	直径5、10、50	1 000	5、10、50	[12]
8	MALDI-QSTAR Pulsar Ⅰ	355	100×150	1 000	100×150	[49]

序号	MALDI仪器	激光波长/nm	激光斑点大小/μm	激光频率/Hz	分辨率/μm	文献
1	ultrafleXtreme MALDI-TOF/TOF质谱仪	355	直径约90	1 000	100	[31]
2	MALDI FlashQuant Workstation	355	100×200	1 000	100或200	[38-39，45]
3	MALDI SYNAPT HDMS	355	100×150	200	150×50	[34]
4	MALDI SolariX FTICR	355	直径30	1 000	500	[25]
5	MALDI SolariX FTICR	355	直径100	25	500	[48]
6	MALDI-QqQ_LIT	355	200×100	1 000	1 000	[27]
7	iMScope TRIO MALDI-IT-TOF	355	直径5、10、50	1 000	5、10、50	[12]
8	MALDI-QSTAR Pulsar Ⅰ	355	100×150	1 000	100×150	[49]

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