法医学杂志 ›› 2022, Vol. 38 ›› Issue (1): 77-81.DOI: 10.12116/j.issn.1004-5619.2021.410611

所属专题: 水中尸体研究专题

• 论著 • 上一篇    下一篇

消解温度与时间对硅藻检验结果的影响

刘景建1(), 杜宇坤2, 赵建3, 康晓东3, 余仲昊2, 郑冬云3, 石河3, 徐曲毅3, 陈立方1,4(), 刘超3()   

  1. 1.昆明医科大学法医学院,云南 昆明 650500
    2.南方医科大学法医学院,广东 广州 510515
    3.广州市刑事科学技术研究所 法医病理学公安部重点实验室,广东 广州 510442
    4.云南公安刑事科学技术研究所,云南 昆明 650228
  • 收稿日期:2021-06-16 出版日期:2022-02-25 发布日期:2022-06-21
  • 通讯作者: 陈立方,刘超
  • 作者简介:刘超,男,博士,主任法医师,主要从事个体识别及死因鉴定;E-mail:liuchaogzf@163.com
    陈立方,男,硕士,主任法医师,主要从事法医病理学及命案现场分析;E-mail:13888715666@126.com
    刘景建(1995—),男,硕士研究生,主要从事法医病理学研究;E-mail:1569877432@qq.com
  • 基金资助:
    广州市科技计划资助项目(2019030001);公安部科技强警基础工作专项资助项目(2020GABJC38)

Effects of Digestive Temperature and Time on Diatom Test

Jing-jian LIU1(), Yu-kun DU2, Jian ZHAO3, Xiao-dong KANG3, Zhong-hao YU2, Dong-yun ZHENG3, He SHI3, Qu-yi XU3, Li-fang CHEN1,4(), Chao LIU3()   

  1. 1.College of Forensic Medicine, Kunming Medical University, Kunming 650500, China
    2.School of Forensic Medicine, Southern Medical University, Guangzhou 510515, China
    3.Guangzhou Forensic Science Institute & Key Laboratory of Forensic Pathology, Ministry of Public Security, Guangzhou 510442, China
    4.Forensic Science Institute of Yunnan Provincial Public Security Department, Kunming 650228, China
  • Received:2021-06-16 Online:2022-02-25 Published:2022-06-21
  • Contact: Li-fang CHEN,Chao LIU

摘要: 目的

探索硅藻检验过程中组织消解温度和消解时间的合理条件。

方法

取肝组织80份,每份2 g,将每份肝组织分别加入2 mL珠江水样混合。设置消解温度为100 ℃、120 ℃、140 ℃、160 ℃、180 ℃和消解时间为40、50、60、70、80 min对所取肝组织和水样混合物进行分组,每组8份。使用微波消解-真空抽滤-扫描电子显微镜法对上述检材进行硅藻检验,检测硅藻回收数量和滤膜上的残留物质量。

结果

消解时间设置为60 min时,不同温度下硅藻的回收数量之间差异有统计学意义(P<0.05),140 ℃时硅藻回收数量最高,可达(28 797.50±6 009.67)个,滤膜上的残留物质量在180 ℃时最低,为(0.60±0.28)mg。消解温度设置为140 ℃时,不同消解时间的硅藻回收数量之间差异具有统计学意义(P<0.05),40 min时硅藻回收数量最高,可达(20 650.88±1 950.29)个,各组滤膜上的残留物质量在不同消解时间组之间差异无统计学意义(P>0.05)。

结论

硅藻消解效果与温度、时间有关,当消解温度为140 ℃,消解时间为40、50、60 min时利于硅藻检出。

关键词: 法医病理学, 硅藻检验, 消解温度, 消解时间

Abstract: Objective

To study the effects of temperature and time for diatoms digestion and find out suitable digestive temperature and time.

Methods

Eighty pieces of liver tissues were collected, each piece of tissue was 2 g, and 2 mL Pearl River water was added to each piece of tissue. The digestion temperature was set at 100 ℃, 120 ℃, 140 ℃, 160 ℃, 180 ℃ and the digestion time was set at 40, 50, 60, 70, 80 min. The liver tissue and water mixture were divided into 8 portions in each group. All the samples were tested by microwave digestive - vacuum filtration - automated scanning electron microscopy method. The quantity of diatom recovered and the quality of residue on the membrane were recorded.

Results

When the digestion time was set to 60 min, there were statistically significant differences in the number of diatoms recovered at different temperatures (P<0.05). The maximum number of diatoms recovered was (28 797.50±6 009.67) at 140 ℃, and the minimum residue was (0.60±0.28) mg at 180 ℃. When the digestion temperature was set at 140 ℃, there were statistically significant differences in the number of diatoms recovered at different digestion times (P<0.05). The number of diatoms recovered was the highest at 40 min, it was up to (20 650.88±1 950.29), and the residue quality of each group had no statistical significance among different digestion time groups(P>0.05).

Conclusion

The effect of diatom digestion is related to temperature and time. When the digestion temperature was 140 ℃ and the digestion time was 40, 50 and 60 min, it is favorable for diatom test.

Key words: forensic pathology, diatom test, digestive temperature, digestive time

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