法医学杂志

法医学杂志 ›› 2010, Vol. 26 ›› Issue (4): 241-245.DOI: 10.3969/j.issn.1004-5619.2010.04.001

• 论著 •    下一篇

小鼠皮肤切创愈合过程中大麻素Ⅰ型受体表达

赵振宾1，官大威1，刘伟伟1，王  涛1，范琰琰1，程子惠2，郑吉龙3，胡更奕1   

  1. （1. 中国医科大学 法医学院，辽宁 沈阳 110001； 2. 中国法医学会 司法鉴定中心，北京 100038； 3. 中国刑事警察学院，辽宁 沈阳 110001）
  • 发布日期:2010-08-25 出版日期:2010-08-28
  • 通讯作者: 通信作者：官大威，男，教授，博士研究生导师，主要从事法医病理学研究；E-mail：dwguan@mail.cmu.edu.cn
  • 作者简介:赵振宾（1983—），男，辽宁本溪人，硕士研究生，主要从事皮肤损伤愈合机制的研究；E-mail：zzb830220@163.com
  • 基金资助:

    教育部高等学校博士学科点专项科研基金项目（200801590020）

Expression of Cannabinoid ReceptorⅠ during Mice Skin Incised Wound Healing Course

ZHAO ZHEN-BIN1, GUAN DA-WEI1, LIU WEI-WEI1, WANG TAO1, FAN YAN-YAN1, CHENG ZI-HUI2, ZHENG JI-LONG3, HU GENG-YI1   

  1. （1. School of Forensic Medicine, China Medical University, Shenyang 110001, China; 2. Forensic Science Identification Center of Chinese Forensic Medicine Association, Beijing 100038, China; 3. China Criminal Police University, Shenyang 110001, China）
  • Online:2010-08-25 Published:2010-08-28

被引次数

1

摘要： 目的 探讨小鼠皮肤切创愈合过程中损伤区大麻素Ⅰ型受体（cannabinoid receptor Ⅰ，CB1R）的表达及不同时间的变化规律。 方法 应用免疫组织化学和Western印迹法检测小鼠皮肤切创后不同时间段CB1R的变化情况。 结果 正常组织中有少量CB1R的表达，位于表皮、毛囊、皮脂腺、皮肌层。伤后6～12 h，中性粒细胞不表达CB1R，伤后1～5 d，阳性染色以单核细胞和成纤维细胞为主。伤后7～14 d，CB1R阳性着色以成纤维细胞为主。阳性细胞率6 h～3 d逐渐升高，5 d达到高峰，7～14 d逐渐降低。经Western印迹法显示各个时间段均有CB1R的阳性条带，其中5 d为CB1R表达的高峰。 结论 在皮肤损伤愈合过程中CB1R被激活，CB1R表达于单核细胞，可能参与炎症反应，CB1R在损伤周边区表达于成纤维细胞，可能参与皮肤损伤后的愈合，其变化规律可用于损伤时间的推断。

关键词: 法医病理学, 伤口愈合, 皮肤, 受体,大麻酚,CB1, 小鼠

Abstract: Objective To investigate the expression of cannabinoid receptor Ⅰ（CB1R） during mice skin incised wound healing course and time-dependent changes of CB1R in wound age determination. Methods The changes of CB1R expression in skin incised wound were detected by immunohistochemistry and Western blotting. Results The control group showed a low expression of CB1R detected mainly in epidermis, hair follicles, sebaceous gland and dermomuscular layer. CB1R expression was undetectable in neutrophils in the wound specimens from 6 h to 12 h post-injury. CB1R positive cells were mostly mononuclear cells（MNCs） and fibroblastic cells（FBCs） from 1 d to 5 d post-injury. CB1R positive cells were mostly FBCs from 7 d to 14 d post-injury. The ratio of the CB1R positive cells increased gradually in the wound specimens from 6 h to 3 d post-injury, reached peak level at 5 d, and then decreased gradually from 7 d to 14 d post-injury. The positive bands of CB1R were observed in all time points of the wound healing course by Western blotting. The expression peak showed at 5 d post-injury. Conclusion CB1R is activated during the wound healing course. The expression of CB1R is found in mononuclear cells, which could be involved in inflammation reaction. CB1R is observed in fibroblastic cells, which could participate in the wound healing. CB1R may be a potentially useful marker for determination of wound healing age.

Key words: forensic pathology, wound healing, skin, receptor, cannabinoid, CB1, mice

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