法医学杂志

法医学杂志 ›› 2011, Vol. 27 ›› Issue (6): 430-433.DOI: 10.3969/j.issn.1004-5619.2011.06.008

• 论著 • 上一篇    下一篇

GC-MS法测定血液中丙泊酚

孙英英1，2，向  平1，刘  伟1，卜  俊1，沈  敏1   

  1. （1. 司法部司法鉴定科学技术研究所 上海市法医学重点实验室，上海 200063； 2. 苏州大学 医学部法医学系，江苏 苏州 215123）
  • 发布日期:2011-12-25 出版日期:2011-12-28
  • 通讯作者: 通信作者：沈敏，女，研究员，博士研究生导师，主要从事法医毒物学研究；E-mail：shenm@ssfjd.cn
  • 作者简介:孙英英（1986—），女，山东威海人，硕士研究生，主要从事法医毒物学研究；E-mail：sunyingying1986@sina.com
  • 基金资助:

    中央级公益性科研院所基本科研业务费专项资金项目（GY0903）；上海市法医学重点实验室资助项目（11DZ2271500）

Determination of Propofol in Human Blood by GC-MS

SUN YING-YING1,2, XIANG PING1, LIU WEI1, BU JUN1, SHEN MIN1   

  1. （1. Shanghai Key Laboratory of Forensic Medicine, Institute of Forensic Science, Ministry of Justice, P.R.China, Shanghai 200063, China; 2. Department of Forensic Medicine, Medical College of Soochow University, Soochow University, Soochow 215123, China）
  • Online:2011-12-25 Published:2011-12-28

摘要： 目的 建立血液中丙泊酚的气相色谱-质谱联用（GC-MS）分析方法。 方法 血液以2，4-二甲基-6-叔丁基苯酚为内标，用乙醚进行液液萃取，离心后取有机层，水浴下挥干，GC-MS检测。 结果 血液中丙泊酚与内标分离良好，在0.02~10 μg/mL范围内线性良好，线性方程为y=0.313 6 x-0.006 8，相关系数为0.999 7，日内精密度与日间精密度（RSD）均小于4.8%，检出限（LOD）为0.005 μg/mL。利用该方法测得实际案例血液中丙泊酚质量浓度为0.14 μg/mL。 结论 建立的GC-MS方法简单、灵敏、可靠，可适用于血液中丙泊酚的法医毒物分析和临床药物监测。

关键词: 法医毒理学, 气相色谱-质谱法, 丙泊酚, 血液

Abstract: Objective To establish a gas chromatography-mass spectrometry （GC-MS） method for determination of propofol in human blood. Methods Propofol and 2-（tert-Butyl）-4,6-dimethylphenol（internal standard） were isolated from human blood samples with liquid-liquid ether extraction. The organic layer was collected after centrifugation and dried using the water bath. The extracted residue was analyzed by GC-MS. Results Propofol and the internal standard showed a good separation with a good linear concentration ranged from 0.02 to 10 μg/mL in blood. The linear function was y=0.313 6 x-0.006 8 with the correlation coefficient of 0.999 7. The precision of intra-day and inter-day were less than 4.8% and the lower limit of detection of propofol was 0.005 μg/mL. Propofol concentration of blood was 0.14 μg/mL using this method in the practice work. Conclusion The GC-MS method is rapid, sensitive, reliable and suitable for qualitative and quantitative analysis propofol of blood in forensic toxicological analysis and clinical drug monitoring.

Key words: forensic toxicology, gas chromatography-mass spectrometry, propofol, blood

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