法医学杂志

法医学杂志 ›› 2014, Vol. 30 ›› Issue (2): 85-87.DOI: 10.3969/j.issn.1004-5619.2014.02.002

• 论著 • 上一篇    下一篇

兔胸部爆炸伤后IL-6、IL-8及TNF-α的时间相关性表达

贺盟1，董利民2，侯晓彬3   

  1. （1. 复旦大学上海医学院法医学系，上海 200032； 2. 上海市公安局闵行分局 刑事科学技术研究所，上海 201199； 3. 中国人民解放军301医院，北京 100083）
  • 发布日期:2014-04-25 出版日期:2014-04-28
  • 作者简介:贺盟（1975—），男，山东临清人，硕士，主检法医师，主要从事法医临床学、法医病理学研究；E-mail：042101055@fudan.edu.cn

Time-related Expression of IL-6、IL-8 and TNF-α Following Explosive Injury to Rabbit’s Chest

HE MENG1, DONG LI-MIN2, HOU XIAO-BIN3   

  1. （1. Department of Forensic Medicine, Shanghai Medical College, Fudan University, Shanghai 200032, China; 2. Institute of Criminal Science and Technology, Minhang Branch of Shanghai Public Security Bureau, Shanghai 201199, China; 3. General Hospital 301 of Chinese PLA, Beijing 100083, China）
  • Online:2014-04-25 Published:2014-04-28

摘要： 目的 探讨细胞因子白细胞介素6（interleukin-6，IL-6）、白细胞介素8（interleukin-8，IL-8）及肿瘤坏死因子α（tumor necrosis factor-α，TNF-α）在兔胸部爆炸伤后的含量变化与损伤时间的关系。 方法 建立家兔胸部爆炸伤的模型，应用酶联免疫吸附法（ELISA）测定不同损伤时间（0.5～12 h）血浆中IL-6、IL-8及TNF-α的表达水平。 结果 IL-6在爆炸伤后3 h开始升高，6 h达峰值；IL-8在爆炸伤后1 h开始升高，6 h达峰值；TNF-α在爆炸伤后0.5 h开始升高，3 h达峰值。 结论 爆炸伤后IL-6、IL-8及TNF-α的蛋白表达水平呈时间相关性。

关键词: 法医病理学；胸部损伤；爆震伤；白细胞介素6；白细胞介素8；肿瘤坏死因子&alpha, ；兔

Abstract: Objective To explore the relationship between the expression changes of cytokines, interleukin-6（IL-6）, interleukin-8（IL-8）, and tumor necrosis factor-α （TNF-α）, and the wound time following explosive injury to rabbit’s chest. Methods The rabbit’s model of explosive injury was established. The expression levels of IL-6、 IL-8 and TNF-α in the plasma were detected by ELISA method at different wound time （0.5-12 h）. Results The level of IL-6 increased at 3 h after wounding and reached peak at 6 h. The level of IL-8 increased at 1 h and reached peak at 6 h. The level of TNF-α increased at 0.5 h and reached peak at 3 h. Conclusion IL-6, IL-8 and TNF-α have a time-related expression after explosive injury.

Key words: forensic pathology, thoracic injuries, blast injuries, IL-6, IL-8, TNF-α, rabbits

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