法医学杂志

法医学杂志 ›› 2013, Vol. 29 ›› Issue (5): 321-324,329.DOI: 10.3969/j.issn.1004-5619.2013.05.001

• 论著 •    下一篇

家兔死后心血氧化还原电位值变化与死亡时间的关系

杨天潼1，于永光2，白  敬2，张海东1，孙婷怡3，Mary G. Ripple4，David R. Fowler4，李  玲1，3，4   

  1. （1. 中国政法大学 教育部证据科学重点实验室，北京 100040； 2. 北京交通大学理学院，北京100044； 3. 华中科技大学同济医学院法医学系，湖北 武汉 430030； 4. 马里兰州法医局，美国 马里兰州 巴尔的摩 21223）
  • 发布日期:2013-10-25 出版日期:2013-10-28
  • 通讯作者: 李玲，女，教授，主要从事法医病理学研究；E-mail：ling001@aol.com
  • 作者简介:杨天潼（1977—），男，吉林长春人，博士，讲师，主要从事法医病理学、法医临床学研究；E-mail：yangtiantong@126.com
  • 基金资助:

    国家自然科学基金青年科学基金资助项目（81001349）；教育部长江学者和创新团队发展计划项目（IRT0956）

Correlation between the Changes of Oxidation Reduction Potential Values and Postmortem Interval of Heart Blood in Rabbits after Death

YANG TIAN-TONG1, YU YONG-GUANG2, BAI JING2, ZHANG HAI-DONG1, SUN TING-YI3, MARY G. RIPPLE4, DAVID R. FOWLER4, LI LING1,3,4   

  1. （1. China University of Political Science and Law, Key Laboratory of Evidence Science, Ministry of Education, Beijing 100040, China; 2. School of Science, Beijing Jiaotong University, Beijing 100044, China; 3. Faculty of Forensic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China; 4. Office of the Chief Medical Examiner of Maryland, Baltimore 21223, USA）
  • Online:2013-10-25 Published:2013-10-28

被引次数

4

摘要： 目的 研究在不同温度条件下家兔死后心血氧化还原电位（oxidation reduction potential，ORP）值变化与死亡时间（postmortem interval，PMI）的关系。 方法 随机将48只家兔分为6组，以空气栓塞法处死后，取家兔右心室血液分别置于10、15、20、25、30和35 ℃温度下的水浴中，自取血即刻（0 h）至死后132 h内，每4 h用PB-21型电化学分析仪测定样本的ORP值。应用SPSS 17.0软件建立曲线回归方程，再应用MATLAB 7.10.0软件建立曲面方程和三维曲面图。 结果 不同温度条件下家兔心血ORP值变化与PMI具有高度相关性，温度高时，ORP值上升明显，温度低时，ORP值上升缓慢，并获得拟合的曲面方程以及三维曲面图。 结论 拟合的ORP值与PMI的曲面方程及三维曲面图可进行温度变化条件下的PMI推断。

关键词: 法医病理学, 死亡时间, 氧化还原电位, 差值函数, 兔

Abstract: Objective To investigate correlation between the changes of oxidation reduction potential （ORP） values of heart blood in rabbits after death and postmortem interval （PMI） at different temperatures. Methods Forty-eight rabbits were randomly divided into 6 groups and sacrificed by air embolism. Blood samples were taken from the right ventricle of each rabbit and stored at different temperatures of 10, 15, 20, 25, 30 and 35 ℃, respectively. Every 4 hours from 0 h to 132 h postmortem, the ORP values of the blood samples were measured at different intervals by PB-21 electrochemical analyzer. The curvilinear regression equation was established by SPSS 17.0 software. The surface equation and 3D surface diagram were established by MATLAB 7.10.0 software. Results The ORP values at different temperatures of heart blood in rabbits were highly correlated with the PMI. The ORP values rised obviously when the temperature was high and rised slowly when the temperature was low. The surface equation and 3D surface diagram were obtained. Conclusion The surface equation and 3D surface diagram of ORP values and PMI may be used for PMI estimation at different temperatures.

Key words: forensic pathology, postmortem interval, oxidation reduction potential, interpolation function, rabbits

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