法医学杂志

法医学杂志 ›› 2015, Vol. 31 ›› Issue (1): 23-27.DOI: 10.3969/j.issn.1004-5619.2015.01.006

• 论著 • 上一篇    下一篇

15个基因座复合扩增体系的建立及新疆维吾尔族遗传多态性

桂  娟1，2，刘海渤3，廖琴香2，徐  旭2，鲁  涤1，2，袁  丽1，2   

  1. （1. 司法文明协同创新中心，北京 100088； 2. 中国政法大学 证据科学教育部重点实验室，北京 100088； 3. 兵团公安局物证鉴定中心，新疆 乌鲁木齐 830000）
  • 发布日期:2015-02-25 出版日期:2015-02-28
  • 通讯作者: 袁丽，女，副教授，主要从事法医生物学研究、鉴定及教学工作；E-mail：yuanliwcy@126.com
  • 作者简介:桂娟（1989—），女，江西鹰潭人，硕士研究生，主要从事法医生物学研究；E-mail：guijuan0926@163.com

Establishment of a 15 Loci Multiplex Amplification System and the Genetic Polymorphism in Xinjiang Uygur Population

GUI JUAN1,2, LIU HAI-BO3, LIAO QIN-XIANG2, XU XU2, LU DI1,2, YUAN LI1,2   

  1. （1. Collaborative Innovation Center of Judicial Civilization, Beijing 100088, China; 2. Key Laboratory of Evidence Science, China University of Political Science and Law, Ministry of Education, Beijing 100088, China; 3. Institute of Forensic Science, Corps Public Security Bureau, Urumqi 830000, China）
  • Online:2015-02-25 Published:2015-02-28

摘要： 目的 建立15个基因座五色荧光复合扩增体系，并调查新疆维吾尔族的遗传多态性。 方法 筛选STR基因座，等位基因测序后按照重复序列重复次数命名，对建立的15个基因座五色荧光复合扩增体系进行灵敏度、种属特异性、同一性及稳定性检测，对新疆维吾尔族群体进行遗传多态性分析，与西藏藏族、岫岩满族、广州汉族进行群体间比较。 结果 建立了15个基因座复合扩增体系，检测灵敏度为0.3 ng，具有良好的种属特异性、同一性和稳定性。新疆维吾尔族13个常染色体STR基因座的基因频率分布符合Hardy-Weinberg平衡，无连锁不平衡现象，大部分基因座在群体间差异具有统计学意义。 结论 建立的体系具有法医物证学应用价值，13个常染色体STR基因座在新疆维吾尔族群体多态性高，适合于亲子鉴定及个人识别，可作为现有基因座的补充。

关键词: 法医遗传学, 多态现象, 遗传, 短串联重复序列, 新疆［维吾尔自治区］, 维吾尔族

Abstract: Objective To develop a five fluorescence-labeled multiplex amplification system for 15 loci and study genetic polymorphism in Xinjiang Uygur population. Methods The STR loci were screened. The alleles were named according to the number of repeats by sequencing. The sensitivity, species specificity, identity and stability of the five fluorescence-labeled multiplex amplification system for the 15 loci were all tested. Then, the genetic polymorphism was analyzed in Xinjiang Uygur population and compared with other ethnic groups including Xizang Tibetan, Xiuyan Manchu, and Guangzhou Han population. Results The 15 loci multiplex amplification system was established. The sensitivity was 0.3 ng with good species specificity, identity and stability. The distributions of genotype for 13 STR loci in Uygur population were in accordance with Hardy-Weinberg equilibrium with no genetic linkage between these loci. Most loci showed statistically significant among different populations. Conclusion The established system has application value in forensic evidence. The 13 STR loci in Uygur population have high polymorphisms to be the supplements to the existing loci.

Key words: forensic genetics, polymorphism, genetic, short tandem repeats, Xinjiang, Uygur nationality

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