法医学杂志

法医学杂志 ›› 2015, Vol. 31 ›› Issue (3): 200-203.DOI: 10.3969/j.issn.1004-5619.2015.03.007

• 论著 • 上一篇    下一篇

尿液中γ-羟基丁酸及其前体物质的检测和应用

施  妍，崔小培，向  平，沈保华   

  1. （司法部司法鉴定科学技术研究所 上海市法医学重点实验室，上海 200063）
  • 发布日期:2015-06-25 出版日期:2015-06-28
  • 通讯作者: 沈保华，男，主任法医师，主要从事法医毒物化学研究；E-mail：shenbh@ssfjd.cn
  • 作者简介:施妍（1984—），女，江苏南通人，博士，主要从事法医毒物化学研究；E-mail：shiy@ssfjd.cn
  • 基金资助:

    上海市青年科技英才扬帆计划项目（14YF1408300）；科研院所社会公益研究专项（GY2014Z-4）；上海市法医学重点实验室项目（14DZ2270800）

Analysis of GHB and Its Precursors in Urine and Their Forensic Application

SHI YAN, CUI XIAO-PEI, XIANG PING, SHEN BAO-HUA   

  1. （Shanghai Key Laboratory of Forensic Medicine, Institute of Forensic Science, Ministry of Justice, P.R.China, Shanghai 200063, China）
  • Online:2015-06-25 Published:2015-06-28

摘要：  目的 建立尿液中γ-羟基丁酸（gamma-hydroxybutyric acid，GHB）及其前体物质1，4-丁二醇（1，4-butanediol，1，4-BD）和γ-丁内酯（gamma-butyrolactone，GBL）的液相色谱-串联质谱法（LC-MS/MS），为相关案件提供依据。 方法 以GHB-d6、MOR-d3为内标，尿样经甲醇沉淀蛋白后通过液相色谱分离，电喷雾离子源进行离子化，多反应监测模式对各化合物进行检测。 结果 GHB及其前体物质1，4-BD、GBL的检出限分别为0.1、0.1和2 μg/mL，准确度为87.6%~98.1%，日内及日间精密度均小于15%，基质效应大于80%。 结论 所建立的分析方法灵敏度高、简便快速、专属性强、可靠性高，可为司法鉴定实践中涉及GHB的案件提供技术支持和基础数据。

关键词: 法医毒理学, 色谱法, 液相, 串联质谱法, &gamma, -羟基丁酸, 尿液

Abstract: Objective To establish the method to analyze γ-hydroxybutyric acid （GHB） and its precursors 1,4-butanediol （1,4-BD） and gamma-butyrolactone （GBL） in urine through LC-MS/MS and provide evidence for related cases. Methods GHB-d6 and MOR-d3 were used as the internal standard. The urine sample was separated by LC after protein precipitation with methanol. The electrospray ion source was for ionization. Each compound was detected through multiple-reaction monitoring （MRM） mode. Results The limits of detection of GHB and its precursors 1,4-BD and GBL were 0.1, 0.1 and 2 μg/mL. The accuracy was 87.6%-98.1%. The intra-day and inter-day precisions were less than 15% and matrix effects were higher than 80%. Conclusion The method is high sensitive, simple, rapid, specific and with high reliability. This study has provided technical support and basic data for forensic cases involving GHB.

Key words: forensic toxicology, chromatography, liquid, tandem mass spectrometry, gamma-hydroxybutyric acid, urine

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