法医学杂志

法医学杂志 ›› 2016, Vol. 32 ›› Issue (1): 1-6.DOI: 10.3969/j.issn.1004-5619.2016.01.001

• 论著 •    下一篇

大鼠尸体细菌演替规律及其在死亡时间推断中的应用（英文）

张  琳1，郭娟娟1，特拉提·赛依提2，彭钰龙1，谢  丹1，郭亚东1，闫  杰1，扎拉嘎白乙拉1，蔡继峰1   

  1. （1. 中南大学基础医学院法医系，湖南 长沙 410013； 2. 新疆警察学院刑侦系，新疆 乌鲁木齐 830011）
  • 发布日期:2016-02-25 出版日期:2016-02-28

Bacterial Succession on Rat Carcasses and Applications for PMI Estimation

ZHANG LIN1, GUO JUAN-JUAN1, T?L?T·SIYIT2, PENG YU-LONG1, XIE DAN1, GUO YA-DONG1, YAN JIE1, ZHA LAGABAIYILA1, CAI JI-FENG1   

  1. （1. Department of Forensic Science, School of Basic Medical Sciences, Central South University, Changsha 410013, China; 2. Department of Investigation, Xinjiang Police College, Urumchi 830011, China）
  • Online:2016-02-25 Published:2016-02-28
  • Contact: CAI Ji-feng, professor, major in forensic pathology and entomology; E-mail: cjf_jifeng@163.com
  • About author:ZHANG Lin （1986—）, postgraduate, major in forensic pathology; E-mail: lyre2008@126.com
  • Supported by:

    the National Natural Science Foundation of China （81373249 and 81571855）, the Science and Technology Committee of Shanghai Municipality （KF1203）, the Fundamental Research Funds for the Central Universities of Central South University （2015zzts276） and the Graduate Student Education Innovation Projects of Central South University （160020009）

摘要： 目的 探讨大鼠尸体细菌演替规律并评估其在死亡时间（PMI）推断中的应用价值。 方法 选取成年雌性SD大鼠，处死后置于纸箱内自然腐败，采集大鼠眼周皮肤、口腔和阴道三个部位的细菌样本，使用纯培养生化方法鉴定菌种，记录菌群构成变化规律。 结果 大鼠三个部位细菌演替规律显示，葡萄球菌属和奈瑟菌属为死后初期的优势菌属，尤其是金黄色葡萄球菌与乳糖奈瑟菌在死后6 h内相对含量较高。干酪乳杆菌规律地出现在死后第3或第4天，且在死后晚期维持较恒定水平。 结论 大鼠死后三个部位的正常细菌及腐败细菌呈现一定的演替规律，可用于PMI推断。

关键词: 法医病理学, 细菌, 死亡时间, 大鼠

Abstract: Abstract: Objective To investigate the bacterial succession on rat carcasses and to evaluate the use of bacterial succession for postmortem interval （PMI） estimation. Methods Adult female SD rat remains were placed in carton boxes. The bacterial colonization of circumocular skin, mouth and vagina was collected to be identified using culture-dependent biochemical methods. The changes in community composition were regularly documented. Results The bacterial succession in three habitats showed that Staphylococcus and Neisseria were predominated in early PMI, especially Staphylococcus aureus and Neisseria lactamica in 6 hours after death. Lactobacillus casei developed on the 3-4 days regularly, and kept stable at a certain level in late PMI. Conclusion The involvement of normal and putrefactive bacteria in three body habitats of rat remains can be used for PMI estimation.

Key words: forensic pathology, bacteria, postmortem interval, rats

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