法医学杂志

法医学杂志 ›› 2016, Vol. 32 ›› Issue (1): 13-17.DOI: 10.3969/j.issn.1004-5619.2016.01.003

• 论著 • 上一篇    下一篇

MALDI-TOF-MS对DAI大鼠脑干蛋白质组学的分析

任冠恒1，2，刘宁国2，陈忆九2，施  妍2，邹冬华2，黄  平2，李正东2，邵  煜2，邓恺飞2   

  1. （1. 南方医科大学基础医学院法医学系，广东 广州 510515； 2. 司法部司法鉴定科学技术研究所 上海市法医学重点实验室，上海 200063）
  • 发布日期:2016-02-25 出版日期:2016-02-28
  • 通讯作者: 刘宁国，男，研究员，主任法医师，主要从事法医病理学研究；E-mail：liuningguo@foxmail.com 陈忆九，男，研究员，博士研究生导师，主要从事法医病理学研究；E-mail：chenyj@ssfid.cn
  • 作者简介:任冠恒（1990—），女，硕士研究生，主要从事法医病理学研究；E-mail：rgh_0101@163.com
  • 基金资助:

    中央级科研院所科研专项（GY2013Z-3、GY2015G-2）

Proteomic Analysis of Rat Brain Stem with DAI by MALDI-TOF-MS

REN GUAN-HENG1,2, LIU NING-GUO2, CHEN YI-JIU2, SHI YAN2, ZOU DONG-HUA2, HUANG PING2, LI ZHENG-DONG2, SHAO YU2, DENG KAI-FEI2   

  1. （1. Department of Forensic Science, School of Basic Medicine Science, Southern Medical University, Guangzhou 510515, China; 2. Shanghai Key Laboratory of Forensic Medicine, Institute of Forensic Science, Ministry of Justice, P.R.China, Shanghai 200063, China）
  • Online:2016-02-25 Published:2016-02-28

摘要： 目的 应用基质辅助激光解吸/电离-飞行时间质谱（matrix-assisted laser desorption/ionization time-of-flight mass spectrometry，MALDI-TOF-MS）技术建立弥漫性轴索损伤（diffuse axonal injury，DAI）诊断模型，筛选DAI相关蛋白或多肽，为寻找DAI的分子标志物提供理论基础。 方法 将15只雄性SD大鼠随机分为DAI组（n=10）和对照组（n=5），采用MALDI-TOF-MS技术对两组大鼠脑干组织的蛋白或多肽表达谱进行检测，运用ClinProTools 2.2分析软件筛选出具有差异的分子峰，应用遗传算法建立诊断模型。 结果 筛选出有差异的分子峰61个（P<0.05），其中DAI组有9个分子峰下调，52个分子峰上调。筛选出5个分子峰建立诊断模型，交叉验证的特异性为96.14%、敏感性为95.98%，盲法验证结果的特异性为73.33%、敏感性为70.00%。 结论 应用MALDI-TOF-MS技术不仅能够建立特异性和敏感性较高的DAI诊断模型，还可以筛选差异蛋白或多肽，为DAI的法医学鉴定奠定理论基础。

关键词: 法医病理学, 弥漫性轴索损伤, 质谱分析法, 基质辅助激光解吸电离, 脑干, 大鼠

Abstract: Objective To establish a diagnostic model for diffuse axonal injury （DAI） by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry （MALDI-TOF-MS）. To screen the proteins or peptides associated with DAI for providing the biomarkers with theoretic foundation. Methods Fifteen male Sprague-Dawley rats were randomly divided into DAI group （n=10） and control group （n=5）. The protein or peptide expression profiles of rat brain stem were detected by MALDI-TOF-MS. ClinProTools 2.2 software was used to find specific peaks, and a diagnostic model was established by the genetic algorithm. Results There were significant differences in 61 peaks of DAI group （P<0.05）, 9 peaks were down-regulated and 52 up-regulated. The diagnostic model was established based on 5 different peaks. The specificity and sensitivity of cross validation was 96.14% and 95.98%; while the specificity and sensitivity of blind validation showed was 73.33% and 70.00%, respectively. Conclusion A specific and sensitive diagnostic model of DAI can be established by MALDI-TOF-MS to provide a potential value for determining DAI in forensic practice.

Key words: forensic pathology, diffuse axonal injury, mass spectrometry, matrix-assisted laser desorption-ionization, brain stem, rats

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