法医学杂志

法医学杂志 ›› 2016, Vol. 32 ›› Issue (2): 94-96.DOI: 10.3969/j.issn.1004-5619.2016.02.004

• 论著 • 上一篇    下一篇

PTEN在冠心病心肌组织中的表达

李雪榕1，何  勇1，雷雨佳1，覃西河1，韦庆涛1，潘新民2，李立娟1，张  林1   

  1. （1. 四川大学华西基础医学与法医学院，四川 成都 610041； 2. 河南科技大学法医学院，河南 洛阳 471003）
  • 发布日期:2016-04-25 出版日期:2016-04-28
  • 通讯作者: 张林，男，教授，主要从事法医遗传学研究；E-mail：zhanglin@scu.edu.cn
  • 作者简介:李雪榕（1991—），女，主要从事法医病理学研究；E-mail：spiderbox@hotmail.com

Expression of PTEN in Myocardial Tissue in Coronary Heart Disease

LI XUE-RONG1, HE YONG1, LEI YU-JIA1, QIN XI-HE1, WEI QING-TAO1, PAN XIN-MIN2, LI LI-JUAN1, ZHANG LIN1   

  1. （1. West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China; 2. School of Forensic Medicine, Henan University of Science and Technology, Luoyang 471003, China）
  • Online:2016-04-25 Published:2016-04-28

摘要： 目的 观察冠状动脉粥样硬化性心脏病（冠心病）患者心肌组织中人第10号染色体缺失的磷酸酶与张力蛋白同源基因（phosphatase and tensin homology deleted on chromosome ten，PTEN）的表达，探讨PTEN的表达与冠心病发生发展的相关性。 方法 选取经病理学确诊为冠心病死亡案例16例作为冠心病组，非心肌病变死亡案例19例作为对照组，采用免疫组织化学以及实时荧光定量PCR法检测心肌组织中PTEN蛋白及其mRNA的表达。 结果 冠心病组PTEN蛋白的表达量低于对照组（P<0.05）；与对照组相比，冠心病组PTEN mRNA表达的差异无统计学意义（P>0.05）。 结论 PTEN可能与冠心病的发生发展有关。

关键词: 法医病理学, 冠状动脉硬化, 心肌, 人第10号染色体缺失的磷酸酶与张力蛋白同源基因

Abstract: Objective To observe the expression of phosphatase and tensin homology deleted on chromosome ten （PTEN） in myocardial tissue in patients with coronary heart disease, and explore the relevance between the expression of PTEN and the occurrence and development of coronary heart disease. Methods A total of 16 death cases with pathological diagnosis of coronary heart disease were collected as experimental group, and 19 cases without myocardial lesions were selected as control group. The expression of PTEN protein and its mRNA were detected by immunohistochemistry and real-time fluorescence quantitative PCR respectively. The correlation between the expression of PTEN and the pathogenesis of coronary heart disease was analyzed. Results The expression of PTEN protein in myocardium in cases with coronary heart disease was significantly lower compared with the control group （P<0.05）. There was no statistical difference of the expression of PTEN mRNA between experimental and control group （P>0.05）. Conclusion PTEN may be involved in the occurrence and development of coronary heart disease.

Key words: forensic pathology, coronary arteriosclerosis, myocardium, phosphatase and tensin homolog deleted on chromosome ten

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