法医学杂志

法医学杂志 ›› 2016, Vol. 32 ›› Issue (6): 401-405.DOI: 10.3969/j.issn.1004-5619.2016.06.001

• 论著 •    下一篇

大鼠急性肾缺血再灌注损伤早期的基因表达

林俊毅1，茅  幸2，吴慧娟2，薛爱民1   

  1. （1. 复旦大学基础医学院法医学系，上海 200032； 2. 复旦大学基础医学院病理学系，上海 200032）
  • 发布日期:2016-12-25 出版日期:2016-12-28
  • 通讯作者: 薛爱民，男，副主任法医师，主要从事法医病理学研究；E-mail：amxue@fudan.edu.cn 吴慧娟，女，讲师，主要从事病理学研究；E-mail：hjwu@shmu.edu.cn
  • 作者简介:林俊毅（1989—），男，博士研究生，主要从事法医病理学研究；E-mail：13111010032@fudan.edu.cn
  • 基金资助:

    上海市科委科创项目（15140902900）

Genes Expression in the Early Stage of Acute Renal Ischemia-reperfusion Injury in Rats

LIN JUN-YI1, MAO XING2, WU HUI-JUAN2, XUE AI-MIN1   

  1. （1. Department of Forensic Medicine, School of Basic Medical Sciences, Fudan University, Shanghai 200032, China; 2. Department of Pathology, School of Basic Medical Sciences, Fudan University, Shanghai 200032, China）
  • Online:2016-12-25 Published:2016-12-28

摘要： 目的 研究急性肾缺血再灌注损伤早期的差异基因表达，探索其潜在的分子机制。 方法 通过单纯夹闭大鼠肾动脉制备肾缺血再灌注模型，进行基因表达芯片检测和生物信息学分析，筛选急性肾损伤早期的差异基因表达及其所涉及的细胞活动和信号通路。同时，通过构建差异表达基因的生物网络来寻找与急性肾损伤关系密切的分子，并进行荧光定量PCR验证。 结果 在检测出的151个差异表达基因中，132个基因显著上调，19个基因显著下调，GO与KEGG通路富集分析结果显示主要与细胞增殖、细胞因子介导的信号通路和免疫反应等有关。荧光定量PCR结果显示，与对照组相比，挑选出的3个与急性肾损伤高度相关的基因（ANXA1、PHLDA1、KLF6）在疾病早期具有显著的表达差异，上升倍数与芯片检测出的倍数基本一致。 结论 本研究揭示了急性肾损伤早期的差异基因表达特征以及所涉及的生物学过程和信号通路，其中ANXA1、PHLDA1、KLF6可能在急性肾损伤的发病机制中起到一定作用。

关键词: 法医病理学, 再灌注损伤, 差异基因表达, 生物信息学

Abstract: Objective To study the differential genes expression in the early stage of acute renal ischemia-reperfusion injury and explore potential molecular mechanisms. Methods The ischemia-reperfusion model was made via clamping renal artery of rat. The microarray detection and bioinformatics analyzing of the genes expression were performed. Differentially expressed genes were screened and related cellular activities and signaling pathways were analyzed in early stage of acute kidney injury. Meanwhile, molecules closely relative to acute kidney injury were explored by establishing a biological network of the differentially expressed genes, and the results were verified by real-time PCR. Results A total of 151 genes showed differential expression in this study, including 132 up-regulated and 19 down-regulated genes. Cell proliferation, cytokines mediated signaling transduction and immune responses were greatly enriched by GO and KEGG analysis. The results of real-time PCR showed that compared with control groups, three selected genes （ANXA1, PHLDA1 and KLF6） which related to the acute kidney injury had an obvious differential expression in the early stage of disease. The multiple of increase was essentially the same as the multiple detected by microarray. Conclusion This study shows differential gene expression profile, related biological processes and signaling pathways involved in the early stage of acute kidney injury. ANXA1, PHLDA1 and KLF6 may play a role in the pathogenesis of acute kidney injury.

Key words: forensic pathology, reperfusion injury, differential gene expression, bioinformatics

中图分类号: