法医学杂志

法医学杂志 ›› 1993, Vol. 0 ›› Issue (4): 151-153+.

• 论文 • 上一篇    下一篇

血中乙醇及其代谢物乙醛、乙酸的顶空气相色谱法测定

吴何坚;沈敏;沈彦;黄仲杰;   

  1. 司法部司法鉴定科学技术研究所,司法部司法鉴定科学技术研究所,司法部司法鉴定科学技术研究所,司法部司法鉴定科学技术研究所 上海 200063,上海 200063,上海 200063,上海 200063
  • 发布日期:1993-08-25 出版日期:1993-08-28

DETERMINATIONS OF ETHANONON AND ITS METABOLITES ACETALDEHYDE AND ACETATE IN BLOOD BY HEAD SPACE GAS CHROMATOGRAPHY.

WU HEJIAN SHEN MIN SHEN YAN HUANG ZHONGJIE.INSTITUTE OF FORENSIC SCIENCES,MINISTRY OF JUSTICE;SHANGHAI.(200063)   

  • Online:1993-08-25 Published:1993-08-28

被引次数

8

摘要： 本文用顶空气相色谱法,在 Porapak S 柱上测定人血中乙醇及其代谢物乙醛、乙酸。全血中乙酸在酸性条件下直接加入甲醇酯化成乙酸甲酯,改善了乙酸的挥发性和色谱行为。方法快速,简便,准确。应用本法测定两例正常人饮酒后血中乙醇、乙醛、乙酸浓度,结果表明血液乙醇浓度在酒后90分钟最高,而血液乙醛、乙酸浓度高峰则与人的乙醇代谢能力有关。

关键词: 乙醇测定, 乙醛测定, 乙酸测定, 顶空气相色谱法

Abstract: Blood samples were analyzed for ethanol,aceta- dehyde and acetate by head space gas chroma- tography using a porapak S column.Acetate was esterified to methyl acetate simply by the addition of acidified methanol.After the oral administration of ethanol to two healthy human subjects,the concentration versus time profiles of blood ethanol, acetadehyde and acetate were determind.The ad- vantage of the method is its speed and simplicity.