法医学杂志

法医学杂志 ›› 2014, Vol. 30 ›› Issue (6): 452-455.DOI: 10.3969/j.issn.1004-5619.2014.06.012

• 论著 • 上一篇    下一篇

采血管中添加剂对血样中乙醇含量的影响

刘冬娴，贺江南   

  1. （湖南警察学院刑事科学技术系，湖南 长沙 410138）
  • 发布日期:2014-12-25 出版日期:2014-12-28
  • 作者简介:刘冬娴（1963—），女，湖南宁乡人，教授，主要从事毒物毒品研究与教学工作；E-mail：liudongxian@aliyun.com

Effects of Additives in Blood Collection Tubes on Testing the Alcohol Concentration in Blood Samples

LIU DONG-XIAN, HE JIANG-NAN   

  1. （Department of Criminal Technology, Hunan Police Academy, Changsha 410138, China）
  • Online:2014-12-25 Published:2014-12-28

被引次数

17

摘要： 目的 探讨不同种类采血管对血样中乙醇含量检测结果的影响。 方法 分别用7种一次性真空采血管［无抗凝剂管、促凝剂管、分离胶-促凝剂管、枸橼酸钠（1∶4）管、枸橼酸钠（1∶9）管、柠檬酸钠（9∶1）管、EDTA-K2管］采集10名志愿者饮酒后2 h血液，用顶空气相色谱法检测血样中乙醇含量。 结果 相同血样用不同的采血管，其乙醇含量检测结果不同，依次为分离胶-促凝剂管>促凝剂管>无抗凝剂管>EDTA-K2管>枸橼酸钠（1∶9）管>枸橼酸钠（1∶4）管，柠檬酸钠（9∶1）管与枸橼酸钠（1∶9）管检测结果基本一致。 结论 采集涉嫌酒后驾驶的驾驶员血样，应选用一次性真空采血管，首选EDTA-K2管。

关键词: 法医毒理学, 乙醇, 血液, 顶空气相色谱法, 添加剂

Abstract: Objective To discuss blood collection tubes with different additives and their effects on the testing results of alcohol concentration in blood samples. Methods Blood samples from 10 volunteers were collected 2 hours after drinking with seven different types of disposable vacuum blood collection tubes, including ordinary tube without anticoagulant, coagulant tube, separating gel-coagulant tube, sodium citrate （1∶4） tube, sodium citrate （1∶9） tube, sodium citrate （9∶1） tube and EDTA-K2 tube. The alcohol concentrations in these blood samples were analyzed by headspace gas chromatography. Results The concentration testing results of the same blood samples in different types of tubes were different from one to another. The sequence was as follows: separating gel-coagulant tube > coagulant tube > ordinary tube without anticoagulant > EDTA-K2 tube > sodium citrate （1∶9） tube > sodium citrate （1∶4） tube, whereas the results of the same blood sample in sodium citrate （1∶9） tube and sodium citrate （9∶1） tube showed no obvious difference. Conclusion It is better to collect a suspicious drunk driver’s blood sample using a disposable vacuum blood collection tube, with the EDTA-K2 tube being preferred.

Key words: forensic toxicology, alcohol, blood, headspace gas chromatography, additive

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