法医学杂志

法医学杂志 ›› 2020, Vol. 36 ›› Issue (5): 682-687.DOI: 10.12116/j.issn.1004-5619.2020.05.014

• 论 著 • 上一篇    下一篇

卤代羟基烷类吸入用麻醉剂对血液中乙醇含量检测的影响

谢冰1 ，闫文静2 ，孟宪跃3 ，苗鑫刚1 ，于峰1 ，董玫1 ，施妍4 ，向平4 ，马春玲1 ，文迪1   

  1. 1. 河北医科大学法医学院 河北省法医学重点实验室 河北省法医分子鉴定协同创新中心，河北 石家庄 050017；2. 河北黄骅法医鉴定中心，河北 黄骅 061100；3. 石家庄市公安局交通管理局，河北 石家庄 050091；4. 司法鉴定科学研究院 上海市法医学重点实验室 司法部司法鉴定重点实验室 上海市司法鉴 定专业技术服务平台，上海 200063
  • 收稿日期:2019-12-04 发布日期:2020-10-25 出版日期:2020-10-28
  • 通讯作者: 文迪，男，博士，教授，副主任法医师，主要从事法医毒理学和毒物分析研究；E-mail：wendi01125@126.com 马春玲，女，博士，教授，主任法医师，主要从事法医毒理学和毒物分析研究；E-mail：chunlingma@126.com
  • 作者简介:谢冰（1982—），女，博士，副研究员，主要从事法医毒理学和毒物分析研究；E-mail：queenie06@163.com
  • 基金资助:
    国家重点研发计划资助项目（2018YFC0807204，2016YFC0800704）

Influence of Halogenated Hydroxyl-Alkanes Inhalation Anesthetic on the Determination of Ethanol Content in Blood

XIE Bing1 , YAN Wen- jing2 , MENG Xian- yue3 , MIAO Xin- gang1 , YU Feng1 , DONG Mei1 , SHI Yan4 , XIANG Ping4 , MA Chun-ling1 , WEN Di1   

  1. 1. Collaborative Innovation Center of Forensic Medical Molecular Identification, Hebei Key Laboratory of Fo? rensic Medicine, College of Forensic Medicine, Hebei Medical University, Shijiazhuang 050017, China; 2. Huang? hua Forensic Identification Center, Huanghua 061100, Hebei Province, China; 3. Shijiazhuang Public Trans? portation Administration Bureau, Shijiazhuang 050091, China; 4. Shanghai Key Laboratory of Forensic Medi? cine, Key Laboratory of Forensic Science, Ministry of Justice, Shanghai Forensic Service Platform, Academy of Forensic Science, Shanghai 200063, China
  • Received:2019-12-04 Online:2020-10-25 Published:2020-10-28

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摘要： 目的 研究血液中的卤代羟基烷类吸入用全身麻醉剂对血液中乙醇含量检测的影响。 方法 利 用顶空气相色谱双柱确认检测方法对卤代羟基烷类进行分析，观察其在KB-BAC1/KB-BAC2和J&W DBALC1/DB-ALC2 气相色谱柱条件下对顶空气相色谱法检测血液中乙醇含量的影响。 结果 J&W DBALC1/DB-ALC2气相色谱柱中，七氟烷、恩氟烷分别与乙醇和叔丁醇的保留时间相近，对血液中乙醇含量 的检测存在干扰；仅J&W DB-ALC1气相色谱柱可分离七氟烷和乙醇成分，以排除其对血液中乙醇含量检 测的影响。在KB-BAC1/KB-BAC2气相色谱柱中，七氟烷、异氟烷与乙醇的保留时间相近，尤其是七氟烷 与乙醇的保留时间基本一致，对血液中乙醇含量检测存在明显干扰。 结论 卤代羟基烷类对顶空气相色 谱法检测血液中乙醇含量存在干扰，选择适当的色谱柱进行双柱确认检测可有效予以甄别。

关键词: 法医毒理学；卤代羟基烷；麻醉药, 吸入；乙醇；顶空气相色谱法；血液

Abstract: Objective To study the influence of halogenated hydroxyl-alkanes inhalation anesthetic on the determination of ethanol content in blood. Methods Halogenated hydroxyl-alkanes were analyzed by headspace gas chromatography with double column confirmatory detection method. The influence of halogenated hydroxyl-alkanes on determination of ethanol content in blood sample by headspace gas chromatography was explored under the different detection conditions of KB-BAC1/ KB-BAC2 and J&W DBALC1/DB-ALC2 gas chromatographic column. Results The retention time of sevoflurane and enflurane was similar to that of ethanol and tert butanol respectively when using the J&W DB-ALC1/DB-ALC2 gas chromatographic column, and interfered with the detection of ethanol content in blood; only J&W DB-ALC1 gas chromatographic column can separate the sevoflurane and ethanol components, so as to eliminate their influence on the detection of ethanol content in blood. When using KB- BAC1/KBBAC2 gas chromatographic column, the retention time of sevoflurane, isoflurane and ethanol is similar, especially that of sevoflurane and ethanol, and sevoflurane obviously interferes with the determination of ethanol content in blood. Conclusion Halogenated hydroxy-alkanes interfere with determination of ethanol content in blood by headspace gas chromatography. The interference can be discriminated effectively by choosing the suitable chromatographic column and double column confirmatory detection

Key words: forensic toxicology, halogenated hydroxy-alkanes, anesthetics, inhalation, ethanol, headspace gas chromatography, blood

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