法医学杂志

法医学杂志 ›› 2005, Vol. 0 ›› Issue (2): 118-120.

• 论文 • 上一篇    下一篇

基体辅助激光解吸电离飞行时间质谱鉴别乌头的研究

潘爱华,刘捷   

  1. 中山大学基础医学院法医系毒物分析室,中山大学基础医学院法医系毒物分析室 广东广州510089 ,广东广州510089
  • 发布日期:2005-04-25 出版日期:2005-04-28

Study on Identification of Aconiti by Matrix-assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry

PAN AI-HUA, LIU JIE (DEPARTMENT OF FORENSIC TOXICOLOGICAL ANALYSIS, PRECLINICAL SCHOOL, SUN YAT-SEN UNIVERSITY,GUANGZHOU 510089,CHINA)   

  • Online:2005-04-25 Published:2005-04-28

被引次数

11

摘要： 目的建立乌头类中药鉴别的基体辅助激光解吸电离飞行时间质谱方法。方法以DHB为基体,优化飞行时间质谱分析条件,对不同种类的乌头类中药进行飞行时间质谱分析,找出乌头类的指纹峰。结果通过比较炮制乌头与生乌头质谱指纹峰的差异,可作出乌头类是否经炮制的判断。结论基体辅助激光解吸电离飞行时间质谱用于乌头类中药的定性鉴别,分析方法灵敏、准确、快速。

关键词: 乌头类, 中药, 基体辅助激光解吸电离, 飞行时间质谱

Abstract: Objective The merhod of determination of traditional Chinese medicine of aconitum root has been established by using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Methods With optimized matrix of 2,5-Dihydroxy- benzoic acid for herbal extract, the identification of aconitum root by MALDI-TOF-MS has been conditioned. By comparison of mass spetra of various of aconitium root ,their fingerprint mass-to-charge ratios were found to perform identification. With the characteristic mass-to-charge ratios ,It was shown that the mass spectrum of raw aconitum roots were obviously different from that of processed aconitum roots. Results The raw radix aconiti could be differentiated from radix aconiti preparate by determination of MALDI-TOF-MS. Conclusion The identifical method of aconitum root by MALDI-TOF-MS is rapid,precise and sensitive,it can be used in toxicological analysis of medical tangle and illegal medical practice.

Key words: aconitum, traditional Chinese medicine, matris assisted laser desorption/ionization, time-of-flight mass spectrometry