法医学杂志

法医学杂志 ›› 2005, Vol. 21 ›› Issue (2): 121-123.

• 论文 • 上一篇    下一篇

离子对SPE-HPLC法检测生物检材中的百草枯

王瑞花,苏少明,秦光明,李绍鹏,柴育芳,刘清   

  1. 昆明医学院法医学院,玉溪市公安局刑科所,昆明医学院法医学院,玉溪市公安局刑科所,玉溪市公安局刑科所,昆明医学院法医学院 云南昆明650031 ,云南玉溪653100 ,云南昆明650031 ,云南玉溪653100 ,云南玉溪653100 ,云南昆明650031
  • 发布日期:2005-04-25 出版日期:2005-04-28

Ion-pair Solid-phase Extraction(SPE) and HPLC Analysis of Paraquat in Biological Sample

WANG RUI-HUA1, SU SHAO-MING2, QIN GUANG-MING1, ET AL. (1. FORENSIC MEDICINE SCHOOL OF KUNMING MEDICAL COLLEGE, KUNMING 650031,CHINA; 2. CRIMINAL SCIENCE AND TECHNOLOGY INSTITUTE OF YUXI PUBLIC SECURITY BUREAU ,YUXI 650031,CHINA)   

  • Online:2005-04-25 Published:2005-04-28

被引次数

106

摘要： 目的建立生物检材中百草枯的简便、快速、灵敏、可靠的高效液相色谱分析方法。方法生物检材酶解后,用以十二烷基三甲基溴化铵和十二烷基硫酸钠预处理过的C18固相柱萃取,HPLC/DAD进行分析。结果回收率81%～94%,检出限为1ng·mL-1,线性范围50ng·mL-1～1mg·mL-1,结论此方法适用于中毒生物检材中百草枯的检测。

关键词: 百草枯, 生物检材, 固相萃取, H, PLC/DAD

Abstract: Objective To establish an HPLC method for the determination of Paraquat in biological samples. Methods Paraquat in biological samples was extracted by C18 columns which were pre-treated with cetyl-trimethyl ammonium bromide(CTAB) and soudium dodecyl sulphate(SDS), and analysed by HPLC/DAD. Results The detection limit of the method was 1ng·mL-1,and the average recoveries were 81%～94%. Conclusion The method can be used to analysis of paraquat in biological samples.

Key words: paraquat, biological sample, ion-pair, solid-phase extraction, HPLC/DAD