法医学杂志

法医学杂志 ›› 2006, Vol. 0 ›› Issue (2): 111-116.

• 论文 • 上一篇    下一篇

多色荧光STR检测试剂盒的研制与验证

李莉;陈光辉;李成涛;柳燕;林源;阙庭志;赵珍敏;王亚新;   

  1. 司法部司法鉴定科学技术研究所,上海申友健海生物技术有限公司,司法部司法鉴定科学技术研究所,司法部司法鉴定科学技术研究所,司法部司法鉴定科学技术研究所,司法部司法鉴定科学技术研究所,司法部司法鉴定科学技术研究所,上海申友健海生物技术有限公司 上海200063,上海201203,上海200063,上海200063,上海200063,上海200063,上海200063,上海201203
  • 发布日期:2006-04-25 出版日期:2006-04-28

Developing of a New Multicolor-fluorescent Labeled STR Amplification Kit

LI LI1, CHEN GUANG-HUI2, LI CHENG-TAO1, LIU YAN1, LIN YUAN1, QUE TING-ZHI1, ZHAO ZHEN-MIN1,WANG YA-XIN2 (1. INSTITUTE OF FORENSIC SCIENCES, MINISTRY OF JUSTICE, P.R.CHINA, SHANGHAI 200063, CHINA; 2. SHANGHAI SHENYOU JIANHAI BIO-TECHNOLOGY CO. LTD, SHANGHA   

  • Online:2006-04-25 Published:2006-04-28

被引次数

14

摘要： 目的建立18个基因座的PCR扩增检测试剂盒。方法采用两个多重PCR体系和四色荧光素标记技术,对Amelogenin和17个STR基因座(D3S1358、vWA、FGA、D8S1179、D21S11、D18S51、D5S818、D13S317、D16S539、TH01、TPOX、CSF1PO、D7S820、D2S1338、D19S433、D12S391和D19S253)进行基因型检测,并对其可靠性、稳定性、效能等进行研究。结果通过2个多重PCR扩增体系,可以成功地对各类生物学检材的Amelogenin和17个STR基因座进行检测,且可靠、稳定、高效。结论本研究所研制的试剂盒,是对该18个基因座进行检测的高效、稳定、可靠的新体系。

关键词: 短串联重复, 多重PCR, 试剂盒开发, 法医学

Abstract: Objective To develop a PCR-based STR system for genotyping of 18 loci (Amelogenin, D3S1358, vWA, FGA, D8S1179, D21S11, D18S51, D5S818, D13S317, D16S539, TH01, TPOX, CSF1PO, D7S820, D2S1338, D19S433, D12S391 and D19S253). Methods By using primers labeled with four color fluorescent (FAM, HEX, TAMRA and ROX), two multiplex amplification reaction systems were developed to genotype Amelogenin and 17 STR loci. Results Amelogenin and these 17 STR loci were genotyped successfully in different kinds of biological samples by the kit. Conclusion The STR amplification kit developed in our study gives a new approach to genotype these 18 loci in a efficient, steady and reliable way.

Key words: short tandem repeat, multiplex PCR, kit developing, forensic medicine