法医学杂志

法医学杂志 ›› 2008, Vol. 0 ›› Issue (1): 1-4.

• 论文 •    下一篇

大鼠急性心肌缺血后脑钠肽、c-fos基因的表达及法医学意义

徐永城;荊小莉;柯咏;王振原;   

  1. 西安交通大学医学院法医学系,西安市公安局高新区分局,西安交通大学医学院法医学系,西安交通大学医学院法医学系 陕西西安710061 宁波市公安局鄞州区分局,浙江宁波315100,陕西西安710075,陕西西安710061,陕西西安710061
  • 发布日期:2008-02-25 出版日期:2008-02-28

Expression of Brain Natriuretic Peptide and c-fos Gene in Rat after Acute Myocardial Ischemia and Its Medicolegal Significance

XU YONG-CHENG1,2, JING XIAO-LI3, KE YONG1, WANG ZHEN-YUAN1 (1. DEPARTMENT OF FORENSIC SCIENCE, MEDICAL COLLEGE, XI′AN JIAOTONG UNIVERSITY, XI′AN 710061, CHINA;2. YINZHOU BRANCH OF NINGBO PUBLIC SECURITY BUREAU, NINGBO 315100, CHINA;3. GAOXIN BRANCH OF XI′   

  • Online:2008-02-25 Published:2008-02-28

被引次数

15

摘要： 目的研究大鼠在急性心肌缺血(acute myocardial ischemia,AMI)后目的基因脑钠肽(brain natriuretic peptide,BNP)和c-fos的mRNA表达变化与AMI发生后所经历时间的关系,为法医病理学鉴定心源性猝死提供依据。方法用结扎法制作大鼠AMI动物模型,结扎成功后观察时间组为10、30、60min和3h,用RT-qPCR、普通PCR检测目的基因BNP和c-fos的mRNA表达变化,同时行HE染色。比较不同时间组间的结果差异。结果RT-qPCR结果显示,BNP在3h组的表达量高于正常组、10min组、30min组及60min组,有显著性差异(P<0.05),c-fos除正常组与10min组之间、30min组与3h组之间无显著性差异(P≥0.05)以外,其他各组间均有显著性差异(P<0.05),并以60min组c-fos基因的表达量最多。普通PCR未见目的基因不同时间的组间差异。HE染色仅在实验组3h组偶见局部嗜酸性增强、肌纤维呈波浪状等改变。结论c-fos基因可能作为缺血发生30min后的辅助诊断指标。RT-qPCR方法较适合早期AMI的诊断。

关键词: 法医病理学, 急性心肌缺血, 脑钠肽, c-fos, mRNA

Abstract: Objective To study the mRNA expression of BNP and c-fos gene in rat heart after acute myocardial ischemia (AMI) and to provide a marker for its medicolegal diagnosis. Methods AMI animal model of rat was made by ligating LAD. mRNA expression of BNP and c-fos gene were studied with RT-qPCR and ordinary PCR at 10 min, 30 min, 60 min and 3 h after the successful ligation. The H&E staining was also used. Changes of the mRNA expression in different time groups were compared. Results There was significant difference in BNP mRNA expression of the 3 h group by RT-qPCR compared with normal control group, 10 min, 30 min, and 60 min groups (P<0.05). There were dramatic differences in c-fos mRNA expression between every two groups (P<0.05) except between the normal group and the 10 min group, between the 30 min group and the 3 h group. The peak of c-fos expression was in 60 min group. No difference was shown between groups by the ordinary PCR. Myocardial fiber acidophilia staining and wavy changes could be seen occasionally at 3 h experimental group by H&E staining. Conclusions C-fos gene probably be used as an auxiliary test for myocardial ischemia of duration of 30 minutes or longer. RT-qPCR may be suitable for diagnosis of early AMI.

Key words: forensic pathology, acute myocardial ischemia, brain natriuretic peptide, c-fos, mRNA