法医学杂志

法医学杂志 ›› 2009, Vol. 0 ›› Issue (1): 1-5.

• 论文 •    下一篇

大鼠脑挫伤后基质金属蛋白酶3的表达

郭晓冲;李如波;梁红霞;王福远;吴旭;   

  1. 中国医科大学法医学院法医病理学教研室;中国医科大学实验动物部辽宁省实验动物转基因重点实验室;
  • 发布日期:2009-02-25 出版日期:2009-02-28

Expression of Matrix Metalloproteinase-3 after Brain Contusion in Rats

GUO XIAO-CHONG1,2, LI RU-BO1, LIANG HONG-XIA1, WANG FU-YUAN1, WU XU1 (1. DEPARTMENT OF FORENSIC PATHOLOGY, SCHOOL OF FORENSIC MEDICINE, CHINA MEDICAL UNIVERSITY, SHENYANG 110001, CHINA; 2. KEY LAB OF LABORATORY TRANSGENIC ANIMAL OF LIAONING PROVINCE, DEPA   

  • Online:2009-02-25 Published:2009-02-28

被引次数

12

摘要： 目的检测脑挫伤修复过程中基质金属蛋白酶3(matrixmetalloproteinase-3,MMP-3)的表达,探讨其表达规律与损伤时间的相关性。方法将大鼠分成对照组、假手术组和脑挫伤组。应用免疫组织化学技术和蛋白质印迹(Westernblot)方法检测脑挫伤后不同时间脑组织中MMP-3的表达变化。结果免疫组化结果:对照组及假手术组脑组织未见MMP-3阳性染色;伤后6h组脑组织出现MMP-3阳性染色,此后24h内染色逐渐增强,伤后5d阳性细胞数及染色强度均达高峰,随之下降,伤后14d仍有少量MMP-3表达。Westernblot结果:对照组及假手术组脑组织未见MMP-3阳性染色;挫伤后6h组出现MMP-3表达,随后表达量逐渐上升,5d达到高峰,以后逐渐下降,14d仍有表达。结论大鼠脑挫伤修复过程中MMP-3的表达呈时序性变化规律,可望用于脑挫伤时间的推断。

关键词: 法医病理学, 脑损伤, 基质金属蛋白酶3, 免疫组织化学, 印迹法, 蛋白质, 损伤时间推断, 大鼠

Abstract: Objective To investigate the expression of matrix metalloproteinase-3 after brain contusion and its applicability for estimating the age of brain contusion. Methods Rats had been divided into three groups: control group, sham operation group and brain contusion group. The expression of matrix metallo- proteinase-3 at different time was detected by immunohistochemistry and Western blot. Results By the immunohistochemistry, no staining was observed in control and sham operation groups. The positive stain- ing of MMP-3 appeared 6 hours after contusion, increased gradually in 24 hours and peaked 5 days after contusion, then started to decrease, 14 days after contusion still could be observed. By the Western blot analysis, no expression of MMP-3 was detected in control and sham groups. The positive staining of MMP-3 appeared 6 hours after contusion, increased gradually and maximized 5 days after contusion, then started to decrease, 14 days after contusion still could be found. Conclusion Time-order expression of MMP-3 could be used for estimating the age of brain contusion in forensic pathology.

Key words: forensic pathology, brain injuries, matrix metalloproteinase-3, immunohistochemistry, blotting, western, wound age estimation, rats