法医学杂志

法医学杂志 ›› 2009, Vol. 0 ›› Issue (2): 102-105.

• 论文 • 上一篇    下一篇

成都汉族群体10个STR基因座的遗传多态性

罗昊;颜静;张林;廖淼;李英碧;侯一平;吴谨;   

  1. 四川大学华西基础医学与法医学院物证教研室;
  • 发布日期:2009-04-25 出版日期:2009-04-28

The Polymorphism of Ten STR loci in Chinese Han Population in Chengdu

LUO HAO, YAN JING, ZHANG LIN, LIAO MIAO, LI YING-BI, HOU YI-PING, WU JIN (DEPARTMENT OF FORENSIC BIOLOGY, WEST CHINA SCHOOL OF PRECLINICAL AND FORENSIC MEDICINE, SICHUAN UNI- VERSITY, CHENGDU 610041, CHINA)   

  • Online:2009-04-25 Published:2009-04-28

被引次数

2

摘要： 目的获得成都汉族群体10个短串联重复序列(short tandem repeat,STR)基因座的群体遗传学数据,评估其法医学应用价值,并对其种属特异性进行研究。方法Chelex法提取100名无亲缘关系的成都汉族个体血液样本的DNA,PCR扩增,非变性聚丙烯酰胺凝胶电泳,硝酸银显色分型。选择10种常见的动物样本进行种属特异性研究,对上述10个基因座的种属特异性进行评价。结果D1S2145、D3S2433、D5S1507、D5S2502、D8S2319、D9S926、D16S767、D17S2181、GATA140E03和GATA196B10基因座在成都汉族群体中等位基因个数分别为6、5、8、5、6、7、7、5、7和7,基因型个数分别为17、14、28、15、16、18、15、14、19和21;等位基因和基因型频率分布均符合Hardy-Weinberg平衡定律。经与10种动物样本比较,D3S2433、D5S1507、D5S2502、D8S2319和GATA196B10有较好的种属特异性,其余基因座分型区内少部分动物有扩增产物。结论上述10个STR基因座在成都汉族群体中具有较好的遗传多态性,在个体识别和亲权鉴定中具有一定的应用价值。

关键词: 法医遗传学, 多态现象, 遗传, 种特异性, 短串联重复序列, 成都, 汉族

Abstract: Objective To obtain data of polymorphism distribution of 10 short tandem repeat (STR) loci D1S2145, D3S2433, D5S1507, D5S2502, D8S2319, D9S926, D16S767, D17S2181, GATA140E03, GA- TA196B10 in Chinese Han population in Chengdu and to evaluate their usefulness in the field of forensic science and their species specificity. Methods DNA of 100 unrelated individuals of Chengdu Han population was extracted with Chelex method, amplified by PCR, then typed with silver staining after polyacry- lamide gel electropho-resis(PAGE). Ten different animals were selected as the controls in this study for evaluating the species specificity of the ten STR loci. Results In the ten STR loci of Chengdu Han population, 6, 5, 8, 5, 6, 7, 7, 5, 7 and 7 alleles were found, respectively. 17, 14, 28, 15, 16, 18, 15, 14, 19 and 21 genotypes were observed in the ten loci, respectively. The allele and genotype frequency distributions of the ten loci were detected no deviation from the Hardy-Weinberg law of equilibrium. By comparison with the data from 10 different animals, the species specificity of D3S2433, D5S1507, D5S2502, D8S2319 and GATA196B10 was good, but part of animals had amplification product at typing field of the other loci. Conclusion The 10 STR loci mentioned above are highly polymorphic and can be used in the forensic personal identification and paternity testing.

Key words: forensic genetics, polymorphism, genetic, species specificity, short tandem repeat, Chengdu, Han nationality