法医学杂志

法医学杂志 ›› 2009, Vol. 0 ›› Issue (4): 267-270.

• 论文 • 上一篇    下一篇

应用常染色体STR基因座共有等位基因数判别全同胞关系

赵书民;李成涛;张素华;李莉;林源;阙庭志;   

  1. 司法部司法鉴定科学技术研究所上海市法医学重点实验室;复旦大学生命科学学院;苏州大学医学部法医学系;
  • 发布日期:2009-08-25 出版日期:2009-08-28

Application of the Number of Allele Shared among Autosomal STR Loci in Full Sibling Identification

ZHAO SHU-MIN1, LI CHENG-TAO1,2, ZHANG SU-HUA3, LI LI1, LIN YUAN1, QUE TING-ZHI1 (1. SHANGHAI KEY LABORATORY OF FORENSIC MEDICINE, INSTITUTE OF FORENSIC SCIENCE, MINISTRY OF JUSTICE, P.R. CHINA, SHANGHAI 200063, CHINA; 2. SCHOOL OF LIFE SCIENCES, FUDAN UNI   

  • Online:2009-08-25 Published:2009-08-28

被引次数

47

摘要： 目的建立基于常染色体STR基因座共有等位基因数的全同胞关系判别标准。方法根据280对全同胞及2003对无关个体Identifiler系统15个STR基因座的分型结果,对15个STR基因座的共有等位基因数(S15)和全同胞指数(FSI)进行统计,应用SAS8.2软件包得出Fisher判别函数并与ITO法结果进行比较。结果全同胞对及无关个体对中共有等位基因数目均符合正态分布。采用Identifiler系统15个STR基因座共有等位基因数进行全同胞关系判别时,判别函数分别为:ZFS=3.26970S15-31.51174和ZUI=1.70058S15-8.52411。用上述判别函数进行全同胞/无关个体关系判别时的平均错判率为0.0298。15个STR基因座共有等位基因数法、CODIS13个STR基因座共有等位基因数法与ITO法判别结果差异无统计学意义。结论应用常染色体STR基因座的共有等位基因数判别全同胞关系简便、可信,易于掌握且不受STR基因座等位基因频率的影响。

关键词: 法医遗传学, 同胞关系, 等位基因, 短串联重复序列, 判别分析, ITO法

Abstract: Objective To establish and evaluate the method of full sibling identification based on the number of allele shared among autosomal STR Loci. Methods Two hundred and eighty full sibling pairs and 2 003 unrelated individual pairs were genotyped in 15 STR loci with Identifiler Kit, and the number of allele shared among the 15 STR loci(S15) and full sibling index(FSI) were calculated. Fisher discriminant functions were established with SAS 8.2 software based on S15, the power of which were compared with ITO method. Results The distribution of S15 in full sibling pair group and unrelated individual pair group were in accord with normal distribution. The established Fisher discriminant functions for each group were ZFS= 3.269 70 S15-31.511 74 and ZUI=1.700 58 S15-8.524 11, respectively. The average error of probability in sibling and unrelated pair group was 0.029 8. There was no statistically significant difference on the power of full sibling discriminant between the method based on the number of allele shared among the 15 STR loci or the CODIS 13 STR loci and the ITO method. Conclusion The method based on the number of allele shared among the 15 STR loci in full sibling identification is convenient, credible, easy to handling and unaffected by the allele frequency of STR loci.

Key words: forensic genetics, sibling relations, alleles, short tandem repeat, discriminant analysis, ITO method