法医学杂志

法医学杂志 ›› 2010, Vol. 0 ›› Issue (1): 6-9.

• 论文 • 上一篇    下一篇

电感耦合等离子体质谱法检测电流损伤皮肤中金属元素

陈圆圆;张建华;马栋;李立;陈忆九;   

  1. 苏州大学医学部法医学系;司法部司法鉴定科学技术研究所上海市法医学重点实验室;复旦大学上海医学院法医学系;
  • 发布日期:2010-02-25 出版日期:2010-02-28

Detection of Metal Elements of Skin in Electric Injury by Inductively Coupled Plasma Mass Spectrometry

CHEN YUAN-YUAN1,2,ZHANG JIAN-HUA2,MA DONG2,LI LI2,3,CHEN YI-JIU2(1.DEPARTMENT OF FORENSIC MEDICINE,MEDICAL COLLEGE OF SOOCHOW UNIVERSITY,SUZHOU 215123,CHINA;2.SHANG-HAI KEY LABORATORY OF FORENSIC MEDICINE,INSTITUTE OF FORENSIC SCIENCE,MINISTRY OF JUSTICE,   

  • Online:2010-02-25 Published:2010-02-28

被引次数

31

摘要： 目的探讨应用电感耦合等离子体质谱(inductively coupled plasma mass spectrometry,ICP-MS)技术检测电流损伤皮肤中金属元素的可行性,建立检测电流损伤皮肤中金属元素的方法。方法用黄铜、紫铜、铝、铁电极材料以220V交流电电击家兔后肢,ICP-MS对电流损伤皮肤中金属元素进行检测。结果与对照组比较:黄铜电击组皮肤中的Cr、Ni、Cu、Zn、Pb含量升高(P<0.05),紫铜电击组皮肤中的Cr、Cu、Pb含量升高(P<0.05),铝电击组皮肤中的Al、Cr、Mn、Co、Ni、Cu、Pb含量升高(P<0.05),铁电击组皮肤中的Cr、Mn、Fe、Ni含量升高(P<0.05)。不同电极材料电击后皮肤中元素种类及含量也存在明显差异。结论ICP-MS可作为检测电流损伤皮肤中金属元素的有效方法,且可应用于触电材料的推断。

关键词: 法医病理学, 电击伤, 皮肤, 电感耦合等离子体质谱仪, 金属化, 兔

Abstract: Objective To explore the feasibility of the detection of metal elements of skin in electric in-jury using inductively coupled plasma mass spectrometry(ICP-MS),and to establish a method to detect metal elements of skin in electric injury.Methods Brass,copper,aluminum and iron were used as elec-trodes to make electrical marks on rabbit hindlimb(220 V AC),and then ICP-MS was used to detect the metal elements in injured skin.Results Comparing with control group,the levels of Cr,Ni,Cu,Zn and Pb showed higher in the group of brass shock(P<0.05);the levels of Cr,Cu and Pb were higher in group of the copper shock(P<0.05);the levels of Al,Cr,Mn,Co,Ni,Cu and Pb displayed stronger in group of aluminum shock(P<0.05);the levels of Cr,Mn,Fe and Ni showed higher in the group of iron shock(P<0.05).The types and levels of metal elements showed the significant difference between different electrode groups after electric injury.Conclusion ICP-MS can be used as an effective tool to determine electric injury,and the method can be used to estimate the features of electrodes.

Key words: forensic pathology, electric injuries, skin, inductively coupled plasma mass spectrometry(ICP-MS), metallization, rabbits