法医学杂志

法医学杂志 ›› 2012, Vol. 28 ›› Issue (1): 12-17.DOI: 10.3969/j.issn.1004-5619.2012.01.003

• 论著 • 上一篇    下一篇

限制性体位对大鼠膈肌生物力学变化的影响

项  剑1，2，关宿东3，闫  骏1，王会云1，岑新海1，宋祥和1，陈守恭1，王  旭2，谷振勇1   

  1. （1. 南通大学医学院法医学系，江苏 南通 226001； 2. 中国政法大学 证据科学教育部重点实验室，北京 100088； 3. 蚌埠医学院生理学教研室，安徽 蚌埠 233060）
  • 发布日期:2012-02-25 出版日期:2012-02-28
  • 通讯作者: 谷振勇，男，博士，教授，博士研究生导师，主要从事血管生物学、创伤性MODS、呼吸肌疲劳的信号转导机制研究；E-mail：zygusz@126.com
  • 作者简介:项剑（1984—），男，浙江瑞安人，硕士，主要从事法医临床学检案工作及相关电生理学研究
  • 基金资助:

    江苏高校优势学科建设工程资助项目（苏财规[2010] 37号）

Effects of Restraint Position on Changes of Diaphragmatic Mechanical Characteristic in Rats

XIANG JIAN1,2, GUAN SU-DONG3, YAN JUN1, WANG HUI-YUN1, CEN XIN-HAI1, SONG XIANG-HE1, CHEN SHOU-GONG1, WANG XU2, GU ZHEN-YONG1   

  1. （1. Department of Forensic Medicine, Medical School of Nantong University, Nantong 226001, China; 2. Key Laboratory of Evidence Science, China University of Political Science and Law, Ministry of Education, Beijing 100088, China; 3. Department of Physiology, Bengbu Medical College, Bengbu 233060, China）
  • Online:2012-02-25 Published:2012-02-28

被引次数

4

摘要： 目的 观察限制性体位对大鼠膈肌生物力学变化的影响，初步探讨一氧化氮（nitric oxide，NO）的作用。 方法 复制限制性体位大鼠模型，实验分对照组、限制性体位12 h组和限制性体位24 h组。检测在体呼吸功能相关指标、离体膈肌条生物力学指标；分光光度法测定血清NO含量；RT-PCR法检测膈肌nNOS和iNOS mRNA表达。 结果 与对照组比较，限制性体位12、24 h组呼吸频率、潮气量及每分通气量均下降；12 h组膈肌条单收缩张力明显降低，低频刺激下张力明显降低，24 h组低频、高频刺激下张力均明显降低；L-NNA预孵育后，膈肌条单收缩张力均增大，24 h组张力-频率关系曲线明显下降；限制性体位12、24 h组血清NO含量明显升高，膈肌nNOS mRNA表达明显上调。 结论 膈肌源性NO抑或循环血液来源的NO参与介导限制性体位引起的膈肌收缩功能降低。

关键词: 法医病理学, 生物力学, 一氧化氮, 体位, 膈肌, 大鼠

Abstract: Objective To observe effects of restraint position on the changes of diaphragmatic mechanical characteristic in rats, and try to explore the role of nitric oxide（NO）. Methods Rat model of restraint position was established. Rats were divided into control group, restraint position 12 h and 24 h groups. The markers of respiratory functions in vivo and the biomechanical markers of diaphragmatic characteristic ex vivo were evaluated. Serum NO levels were measured with spectrophotometry. The expressions of nNOS and iNOS mRNA in diaphragm were detected using RT-PCR. Results Compared with control group, respiratory rate, tidal volume and minute ventilation were significantly decreased in the restraint position 12 h and 24 h groups. Pt of diaphragm significantly decreased and force-generating capacity reduced at low frequency stimulation in 12 h group. Force-generating capacity over the full range reduced at low and high frequency stimulation in 24 h group. Pt of diaphragm in control and restraint position groups increased after L-NNA pre-incubation. Force-frequency relationship after L-NNA pre-incubation reduced in 24 h group. NO level in serum increased significantly in the restraint position groups. Diaphragmatic nNOS mRNA expression was upregulated significantly in the restraint position groups. Conclusion Restraint position induces the decreasement of diaphragmatic contractility and the decreasement is mediated by NO from diaphragm or circulation blood.

Key words: forensic pathology, biomechanics, nitric oxide, position, diaphragm, rats

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