法医学杂志

法医学杂志 ›› 2015, Vol. 31 ›› Issue (4): 269-272.DOI: 10.3969/j.issn.1004-5619.2015.04.004

• 论著 • 上一篇    下一篇

福建畲族人群26个Y-STR基因座遗传多态性及其法医学应用

边英男1，特来提·赛依提2，朱如心1，赵  琪1，3，张素华1   

  1. （1. 司法部司法鉴定科学技术研究所 上海市法医学重点实验室，上海 200063； 2. 新疆警察学院，新疆 乌鲁木齐 830011； 3. 苏州大学医学部法医学系，江苏 苏州 215123）
  • 发布日期:2015-08-25 出版日期:2015-08-28
  • 作者简介:边英男（1981—），男，辽宁沈阳人，博士，助理研究员，主要从事法医遗传学研究；E-mail：bianyingnan@yeah.net
  • 基金资助:

    国家自然科学基金资助项目（81302620）；国家“十二五”科技支撑计划项目（2012BAK16B01）；中央级公益性科研院所项目（GY2015G4）

Genetic Polymorphisms of 26 Y-STR Loci in Fujian She Nationality and Its Forensic Application

（1. SHANGHAI KEY LABORATORY OF FORENSIC MEDICINE, INSTITUTE OF FORENSIC SCIENCE, MINISTRY OF JUSTICE, P.R.CHINA, SHANGHAI 200063, CHINA; 2. XINJIANG POLICE COLLEGE, URUMQI 830011, CHINA; 3. DEPARTMENT OF FORENSIC MEDICINE, MEDICAL COLLEGE OF SOOCHOW UNIVERSITY, SUZHOU 215123, CHINA）   

  1. BIAN Ying-nan1, TeleT·SIYIT2, ZHU Ru-xin1, ZHAO Qi1,3, ZHANG Su-hua1
  • Online:2015-08-25 Published:2015-08-28

被引次数

2

摘要： 目的 对GoldeneyeTM DNA身份鉴定系统26Y试剂盒在畲族人群中的法医学应用进行研究。 方法 应用毛细管电泳分型技术，对53名福建地区畲族男性无关个体的26个Y-STR基因座进行检测，获得基因型分布状况，计算等位基因频率及单倍型多样性等群体遗传学参数，并对福建畲族与其他民族群体遗传关系进行分析。 结果 53名无关个体中的26个Y-STR基因座共观察到126个等位基因，等位基因频率为0.010 1~0.886 8。26个Y-STR基因座的GD值为0.211 2~0.846 2，有19个基因座的GD值大于0.5。共观察到47种单倍型。根据RST构建的遗传距离矩阵显示福建畲族与闽南汉族之间遗传距离最近，其次是南方汉族，与北方民族的遗传距离相对较远。 结论 GoldeneyeTM DNA身份鉴定系统26Y试剂盒所包含的26个Y-STR基因座在福建畲族中多态性较好，在特殊案件中可作为良好的补充体系，具有法医学应用价值。

关键词: 法医遗传学, 多态现象, 遗传, Y染色体, 短串联重复序列, 畲族, 福建

Abstract: Objective To study the forensic application of GoldeneyeTM DNA ID 26Y Kit in the She nationality. Methods Through capillary electrophoresis, the genotype of 26 Y-STR loci were analyzed in 53 unrelated male individuals from Fujian She nationality. The population genetics parameters such as allele frequency and haplotype diversity were calculated. The comparisons among the She nationality and the other nationalities were analyzed. Results A total of 126 alleles were observed on the 26 Y-STR loci of 53 unrelated male individuals. The allele frequencies and GD value ranged from 0.010 1 to 0.886 8 and 0.211 2 to 0.846 2, respectively. The GD value was greater than 0.5 in the 19 loci. A total of 47 haplotypes were observed. Based on RST, multidimensional scaling plot indicated that the genetic relationship among Fujian She nationality and Minnan Han nationality was closest, followed by Southern China Han nationality and Northern China nationality. Conclusion GoldeneyeTM DNA ID 26Y Kit including 26 Y-STR loci has good polymorphism in the She nationality. As an additional system, it has forensic application value in some special cases.

Key words: forensic genetics, polymorphism, genetics, Y chromosome, short tandem repeat, She nationality, Fujian

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