法医学杂志

法医学杂志 ›› 2026, Vol. 42 ›› Issue (4): 345-351.DOI: 10.12116/j.issn.1004-5619.2024.540103

• 技术与应用 • 上一篇    

八色荧光标记mini-STR复合扩增体系的构建及法医学应用

王鑫1(), 崔扬1, 宣金锋2, 陈维忠1, 张健1, 周如华1(), 史明皓3, 陈林丽3   

  1. 1.苏州市公安局物证鉴定中心，江苏 苏州 215131
    2.中国医科大学法医学院，辽宁 沈阳 110122
    3.江苏安科华捷生物科技有限公司，江苏 无锡 214174
  • 收稿日期:2024-01-10 发布日期:2026-10-28 出版日期:2026-08-25
  • 通讯作者: 周如华
  • 作者简介:王鑫（1986—），男，硕士，警务技术副高级任职资格，主要从事法医物证DNA检验相关研究；E-mail：wangxin5552137@163.com
  • 基金资助:
    苏州市科学技术局社会发展科技创新专项（应用基础研究）资助项目(SS202140)

Development of a Multiplex PCR Amplification System of mini‑STR with Eight‑Dye Fluorescence and Its Application in Forensic Medicine

Xin Wang1(), Yang Cui1, Jinfeng Xuan2, Weizhong Chen1, Jian Zhang1, Ruhua Zhou1(), Minghao Shi3, Linli Chen3   

  1. 1.Institute of Forensic Science, Suzhou Public Security Bureau, Suzhou 215131, Jiangsu Province, China
    2.School of Forensic Medicine, China Medical University, Shenyang 110122, China
    3.Jiangsu AnkeHugen Bio?Technology Co. , Ltd. , Wuxi 214174, Jiangsu Province, China
  • Received:2024-01-10 Online:2026-10-28 Published:2026-08-25
  • Contact: Ruhua Zhou

RichHTML

0

PDF (PC)

132

补充材料

1

摘要：

目的 利用mini-STR与八色荧光标记技术，建立八色荧光复合扩增体系，并评价其在降解样本检测中的应用价值。 方法 采用八色荧光标记技术，对26个基因座（D22S1045、D13S317、CSF1PO、D2S441、D10S1248、TPOX、D16S539、D5S818、Penta D、TH01、D2S1338、Penta E、rs2032678、Amelogenin、D19S433、D21S11、D6S1043、D8S1179、vWA、D7S820、D1S1656、DYS391、D18S51、D12S391、D3S1358、FGA）进行复合扩增体系的构建，并对所构建的复合扩增体系进行特异性、灵敏度、准确性及降解样本检测能力等方面性能的评价与测试。 结果 开发出一款包含26个基因座的八色荧光复合扩增体系，所有基因座扩增子都在310 bp以内，具有特异性好、灵敏度高、分型准确等优点，在降解样本的检测中具有良好的效果。 结论 mini-STR结合八色荧光构建的复合扩增体系对降解样本的检测具有良好应用价值，可以用于法医学实践。

关键词: 法医遗传学, 短串联重复序列, 八色荧光, 复合扩增, 降解样本

Abstract:

Objective To develop an eight-dye fluorescent multiplex amplification system based on mini-STR and eight-dye fluorescent labeling technique, and to evaluate its applicability in the analysis of degraded samples in forensic medicine. Methods An eight-dye fluorescent labeling technique was used to develop a multiplex amplification system targeting 26 loci, including D22S1045, D13S317, CSF1PO, D2S441, D10S1248, TPOX, D16S539, D5S818, Penta D, TH01, D2S1338, Penta E, rs2032678,Amelogenin, D19S433, D21S11, D6S1043, D8S1179, vWA, D7S820, D1S1656, DYS391, D18S51, D12S391, D3S1358, and FGA. The established amplification system was evaluated and tested for its specificity, sensitivity, accuracy, and the ability to analyze degraded samples. Results An eight-dye fluorescent multiplex amplification system containing 26 loci was successfully developed. All amplicons were less than 310 bp in length. The system demonstrated high specificity, sensitivity, and accurate typing, and performed well in the analysis of degraded samples. Conclusion The mini-STR with the eight-dye fluorescent multiplex amplification system has good potential for the analysis of degraded samples and can be applied to forensic casework.

Key words: forensic genetics, short tandem repeat, eight-dye fluorescence, multiplex amplification, degraded sample

中图分类号: