法医学杂志

法医学杂志 ›› 1995, Vol. 0 ›› Issue (3): 0-0.

• 论文 •    

DNA指纹技术在亲子鉴定中的应用

徐苏云，邹苹，徐和平，李家勇，李正坚，邹浪萍，杨树华   

  1. 云南省高级人民法院鉴定科学技术研究所，昆明市妇幼保健院，云南省人民医院
  • 发布日期:1995-06-25 出版日期:1995-06-28

APPLICATION OF DNA FINGERPRINT PATTERNS IN PATERTTY TEST

XU SUYUN;ZOU PING; ET AL(INSTITUTE OF FORENSIC SCIENCES, HIGHER PEOPLE'S COURT OF YUNNAN PROVIN CE; KUNMING 650021)   

  • Online:1995-06-25 Published:1995-06-28

被引次数

32

摘要： 用非放射性标记α－珠蛋白－３＇ＨＶＲ探针在低强度洗涤条件下，对人的ＤＮＡ指纹进行了分析，获得了清晰易读的ＤＮＡ指纹图。并用该方法调查了云南省的７８名无关个体，经统计学分析，计算出无关个体的相关机率是２．４×１０－４。用该方法进行亲子鉴定，获得了满意的结果，用ＨｉｎｆⅠ和ＨｅａⅢ两种内切酶酶切，解决了子代陌生带问题，用该方法对二十余起亲权纠纷案进行了鉴定，证明该方法稳定、结果可靠，父权肯定机率均在９９．９％以上。

关键词: DNA指纹图, 探针, 亲子鉴定

Abstract: In the circumstances of low-stringency-washing, the authors have got clear and high distinctive patterns of human DNA fingerprints by using nonradioactive labelled probe α-globulin-3' HVR. The DNA fingerprints of 78 unrelated individuals living in Yunnan Province of China were de tected and the probability of association between random individuals was calcalated as 2.4×10-14. the DNA finger printing technique was applied in cases of disputed peternity and proved to be stable and reliable.

Key words: DNA, probe, paternity test