法医学杂志

法医学杂志 ›› 2003, Vol. 0 ›› Issue (1): 1-3.

• 论文 •    下一篇

重度颅脑损伤后GDNF表达的实验研究

王欣,廖志钢,刘敏,张林,易旭夫,胡华子   

  1. 四川大学华西基础医学与法医学院,四川大学华西基础医学与法医学院,四川大学华西基础医学与法医学院,四川大学华西基础医学与法医学院,四川大学华西基础医学与法医学院,四川大学华西基础医学与法医学院 四川 成都610041 ,四川 成都610041 ,四川 成都610041 ,四川 成都610041 ,四川 成都610041 ,四川 成都610041
  • 发布日期:2003-02-25 出版日期:2003-02-28

GDNF expression following the severe brain injury in rats

WANG XIN, L1AO ZHI-GANG, LIU MIN, ET AL. (FORENSIC PATHOLOGY DEPARTMENT, SCHOOL OF LEGAL MEDICINE, SICHUAN UNIVERSITY, CHENGDU 610041)   

  • Online:2003-02-25 Published:2003-02-28

摘要： 目的观察重度颅脑损伤后GDNF的表达变化规律。 方法应用免疫组织化学方法和图像分析技术,对大鼠重度颅脑损伤后不同时间大脑皮层、丘脑和脑干等几个脑区内GDNF的表达变化进行研究。结果CDNF在脑损伤后1d表达开始增高,3d达表达最高峰,5d时回落不明显,7d有所回落,但仍高于正常水平。结论GDNF在脑损伤后的时序性变化规律使其有可能成为一种脑损伤时间推断的客观指标。

关键词: 胶质细胞源性神经营养因子(GDNF), 免疫组织化学, 颅脑损伤

Abstract: Objective To evaluate the changes of GDNF after severe brain injury. Methods Changes GDNF in cortex, thalamus and pontine was observed by using immunohistochemistry and image analysis technique. Results The lever of GDNF was increasing at Id, reaching peak at 3d, not obviously decreasing at 5d and still higher than normal at 7d after severe brain injury. Conclusion As an objective indication, the time sequence regularity of GDNF after brain injury may be applied in brain injury time estimation.

Key words: glial cell line-derived neurotrophic factor, immunohistochemistry, brain injury