高效液相色谱法分离测定药酒中乌头碱的含量

法医学杂志 ›› 2005, Vol. 0 ›› Issue (1): 34-35+3.

高效液相色谱法分离测定药酒中乌头碱的含量

李红海,余陆生,金鸣

岳阳市公安局刑警支队,岳阳市公安局刑警支队,华中科技大学同济医学院湖南岳阳414000 ,湖南岳阳414000 ,湖北武汉430030

发布日期:2005-02-25 出版日期:2005-02-28

Separation and Determination of Aconitine in Medicine Wine by Reversed-Phase High Performance Liquid Chromatography

LI HONG-HAI1, YU LIU-SHENG1, JIN MING2 (DEPARTMENT OF FORENSIC MEDICINE, BUREAU OF YUEYANG PUBLIC SECURITY, YUEYANG 414000,CHINA; 2. TONGJI MEDICAL COLLEGE, HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY,WUHAN 430030,CHINA)

Online:2005-02-25 Published:2005-02-28

摘要/Abstract

摘要： 目的建立药酒中乌头碱的高效液相色谱快速分析方法。方法以白酒添加乌头碱对照品对药酒样品的前处理方法、仪器测试条件、线性范围、精密度、回收率进行全面考察,建立定量分析方法。对药酒中所含乌头碱的浓度进行测定。结果该方法乌头碱在药酒中的线性范围是0.45～9.0μg·mL-1;日内、日间精密度分别小于3.1%和4.7%;回收率在(97.3±2.8)%以上。结论所建方法实用、便捷,可对药酒中乌头碱的含量进行快速检测。

关键词: 法医毒物分析, 乌头碱, 药酒, 高效液相色谱

Abstract: Objective For the purpose of more accurate and rapid analysis of aconitine in medicine wine, a reversed-phase High Performance Liquid Chromatography (HPLC) method was developed. Methods Standard aconitine was added to the blank wine when the wine sample was pretreated. The pretreatment method of samples, the linear range, the precision, the recoveries in the plasma were investigated by using of white wine plasma spiked with standard aconitine. Results The linear range was 0.45～9.0 μg·mL-1, r=0.998 8. The detective limit was 0.45 μg·mL-1, The intra and inter-day precision of assay for aconitine were less than 3.1% and 4.7% (n=5) in wine respectively. The recoveries of aconitine were more (97.3±2.8)% in medicine wine. The HPLC method has been used to investigate the concentration of aconitine in one forensic medicine case. Conclusion The HPLC method of quantitative analysis aconitine is rapid and sensitive. It is only in the 2.0 h that determination of aconitine in the medicine wine.

Key words: forensic toxicological analysis, aconitine, medicine wine, high-performance liquid chromatography

李红海;余陆生;金鸣. 高效液相色谱法分离测定药酒中乌头碱的含量[J]. 法医学杂志, 2005, 0(1): 34-35+3.

LI HONG-HAI;YU LIU-SHENG;JIN MING (DEPARTMENT OF FORENSIC MEDICINE;BUREAU OF YUEYANG PUBLIC SECURITY;YUEYANG 000;CHINA;. TONGJI MEDICAL COLLEGE;HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY;WUHAN 000;CHINA). Separation and Determination of Aconitine in Medicine Wine by Reversed-Phase High Performance Liquid Chromatography[J]. , 2005, 0(1): 34-35+3.

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