法医学杂志

法医学杂志 ›› 2005, Vol. 0 ›› Issue (1): 36-38.

• 论文 • 上一篇    下一篇

体液中微量士的宁的富集及同时检测

张静,贺浪冲,傅强   

  1. 西安交通大学医学院,西安交通大学医学院,西安交通大学医学院 陕西西安710061 ,陕西西安710061 ,陕西西安710061
  • 发布日期:2005-02-25 出版日期:2005-02-28

Concentration and Determination of Strychnine Alkaloid in Biological Fluids

ZHANG JING, HE LANG-CHONG.FU QIANG (SCHOOL OF MEDICINE, XI′AN JIAOTONG UNIVERSITY, XI′AN 710061, CHINA)   

  • Online:2005-02-25 Published:2005-02-28

被引次数

22

摘要： 目的将分子印迹技术运用到法医毒物分析中,建立一种全新的测定体液中微量士的宁的方法。方法运用分子印迹原位聚合技术,在色谱柱中一步合成了对士的宁具有专属识别能力的分子印迹聚合物,以此聚合物为介质,装填一厘米的小柱,建立体液中微量士的宁的富集及同时检测方法。结果以士的宁分子印迹聚合物作为尿液和血浆中微量士的宁的富集和检测介质,方法的检出限为4.9ng,样品回收率均大于92%,相对标准偏差小于6.59%,工作曲线的线性相关系数分别为0.9991和0.9966。运用此方法对中毒家兔血浆和尿液中的士的宁进行了测定。结论建立的新方法可以实现对士的宁专属的、灵敏的检测,适合于法医学毒物分析运用。

关键词: 分子印迹, 士的宁, 富集

Abstract: Objective To establish a new method for determination of strychnine alkaloid in biological fluids based on molecularly imprinted polymers. Methods A strychnine molecularly imprinted monolithic column was prepared by in-situ molecularly imprinted technique. The polymer was filled to a 1cm column, and a method was developed to concentrate and determine strychnine alkaloids in biological fluids. Results The limit of detection of the method was 4.9 ng, and the recoveries were more than 92%. The relative standard deviations were smaller than 6.59%. The linear correlation coefficients of standard curves were 0.999 1 and 0.996 6 respectively. This method was applied to concentrate and determine strychnine in plasma and urine of poisoned rabbit. Conclusion The new method could concentrate and simultaneously determine strychnine alkaloids in biological fluids, and it was applied to forensic toxicological analysis.

Key words: molecularly imprinted technique, strychnine, accumulation