法医学杂志

法医学杂志 ›› 2006, Vol. 0 ›› Issue (3): 207-209.

• 论文 • 上一篇    下一篇

广东汉族DXS10011和DXS8377单体型

吕德坚;陈树春;王学文;刘秋玲;陆惠玲;   

  1. 中山大学基础医学院法医物证教研室,中山大学基础医学院法医物证教研室,中山大学基础医学院法医物证教研室,中山大学基础医学院法医物证教研室,中山大学基础医学院法医物证教研室 广东广州510089,广东广州510089,广东广州510089,广东广州510089,广东广州510089
  • 发布日期:2006-06-25 出版日期:2006-06-28

Haplotypes of DXS10011 and DXS8377 in Guangdong Han Individuals

LU DE-JIAN, CHEN SHU-CHUN, WANG XUE-WEN, LIU QIU-LING, LU HUI-LING(DEPARTMENT OF FORENSIC SEROLOGY, ZHONGSHAN-MEDICAL COLLEGEL OF SUN YAT-SEN UNIVERSITY, GUANGZHOU 510089, CHINA)   

  • Online:2006-06-25 Published:2006-06-28

被引次数

5

摘要： 目的调查DX10011和DXS8377两个X染色体STR在广东汉族男性群体中的遗传多态性。方法DNA样本来自广东汉族男性无关个体,PCR后用聚丙烯酰胺凝胶电泳和银染法进行DNA分型。结果在113名个体中,DXS10011和DXS8377分别检出20和12个等位基因。DXS10011-DXS8377共有72种不同的单体型,最多见的单体型只出现了3次。在没有母亲时,DXS10011-DXS8377的女孩的非父排除率为0.9588。在83个孩子为女孩的三联体家系和29个有两个孩子的家系中,DXS10011和DXS8377均表现为共显性连锁遗传,且没有发现突变。结论本数据表明DXS10011和DXS8377在复杂的亲权鉴定中可提供高的遗传多态性信息。

关键词: 短串联重复, X染色体, 单体型, 亲权鉴定

Abstract: Objective To investigate genetic polymorphism of two X chromosome specific STR: DXS10011 and DXS8377 in male samples from Guangdong Han population. Methods The DNA samples were amplified by PCR and analyzed by polyacrylamide gel electrophoresis followed by silver staining. Results Among 113 samples, 20 alleles were found for DXS1011 and 12 alleles for DXS8377. Also, 72 DXS10011-DXS8377 haplotypes were shown. The most common haplotypes only occurred three times. When only female children were tested in motherless case, the exclusion probability of paternity was 0.9588 for DXS10011-DXS8377 haplotypes. Investigations in 83 family trios with female children and 29 pedigrees with two children suggested a co-dominant X-linked inheritance; mutations were not found. Conclusion Our data indicate that DXS10011 and DXS8377 are highly informative X chromosome markers for complicated kinship analysis.

Key words: short tandem repeat(STR), X chromosome, haplotype, paternity testing