法医学杂志

法医学杂志 ›› 2006, Vol. 0 ›› Issue (4): 248-250.

• 论文 • 上一篇    下一篇

挤压家兔后肢导致急性肝损伤和抗氧化能力降低

韩业兴;谷振勇;丛斌;岑新海;刘东旭;刘晓;   

  1. 苏州大学医学院法医学教研室,苏州大学医学院法医学教研室,河北医科大学法医学系,河北医科大学法医学系,河北医科大学法医学系,张家口市公安局刑警支队 江苏苏州215123,江苏苏州215123,河北石家庄050017,河北石家庄050017,河北石家庄050017,河北张家口075000
  • 发布日期:2006-08-25 出版日期:2006-08-28

Mechanism of Acute live Injury Induced by Crushing Hindlimbs in Rabbits

HAN YE-XING1, GU ZHEN-YONG1, CONG BIN2, CEN XIN-HAI2, LIU DONG-XU2, LIU XIAO3 (1.DEPARTMENT OF FORENSIC MEDICINE, MEDICAL SCHOOL OF SOOCHOW UNIVERSITY, SUZHOU 215123, CHINA; 2.DEPARTMENT OF FORENSIC MEDICINE, HEBEI MEDICAL UNIVERSITY, SHIJIAZHUANG 050017,   

  • Online:2006-08-25 Published:2006-08-28

摘要： 目的本实验观察挤压家兔后肢导致肝氧化损伤,探讨氧化应激在挤压家兔后肢导致肝损伤中的作用。方法用标准重物间断压迫家兔后肢复制模型。采用生化方法检测ALT和AST活性;分析天平称重方法检测肝脏湿干重比值(W/D);比色法测定肝组织超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)活性和总抗氧化能力(T-AOC)以及丙二醛(MDA)含量变化;并观察肝组织病理学改变。结果血清ALT和AST活性明显增高(P<0.01),肝细胞明显肿胀,肝窦轻度淤血,而肝脏湿干重比值(W/D)轻度增加(P<0.05);肝脏氧化损伤明显加重,MDA含量增高,而肝脏抗氧化能力下降,SOD、CAT、GSH-Px活性和T-AOC分别比对照组降低了17%、29%、24%和21%(P均<0.01)。结论挤压家兔后肢引起急性肝损伤及肝脏的抗氧化能力下降;肝脏局部抗氧化能力下降可能是肝损伤的重要因素。

关键词: 肝脏, 氧化应激, 抗氧化能力, 挤压伤

Abstract: Objective To investigate the role of oxidative stress in acute liver injury during crushing hind-limbs in rabbit. Methods The crushing injury model in rabbit was established by intermittent crushing the hind limbs of rabbit with standard weight. The ALT and AST activities were spectrophotometrically measured. The weight ratio (wet/dry,W/D) of livers was measured with scale, and the pathologic changes were observed. Superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) and total anti-oxidant capacity (T-AOC) as well as malondialdehyde(MDA) level were spectrophotometrically measured. Results As compared with control rabbits, crushing hindlimbs of rabbits induced acute liver injury with the increase in ALT and AST activities in serum,which were 4.31 (P<0.01) and 10.54 times (P<0.01) of control group respectively, there were cellular swellings and slight congestion of hepatic sinuses. In addition,crushing hind-limbs elicited significant decrease in SOD,CAT,GSH-Px activity and T-AOC to 17%,29%,24% and 21%(P<0.01)compared with control group respectively, whereas MDA level markedly enhanced. Conclusion Crushing hindlimbs of rabbits induced acute liver injury and significant decrease in anti-oxidant capacity, the latter maybe play an important role in crushing hind-limbs of rabbits-elicited the acute liver injury.

Key words: liver, oxidative stress, antioxidant capacity, crushing injury