法医学杂志

法医学杂志 ›› 2014, Vol. 30 ›› Issue (4): 250-252.DOI: 10.3969/j.issn.1004-5619.2014.04.003

• 论著 • 上一篇    下一篇

大鼠软组织挤压伤后NO对肝组织TNF-α和IL-1β基因表达的影响

董国凯1，2，3，张晓彤2，马丽琴2，李  娜2，马春玲2，丛  斌2，谷振勇1，2   

  1. （1. 南通大学医学院法医学系，江苏 南通 226001； 2. 河北医科大学法医学系，河北 石家庄 050017； 3. 徐州医学院法医学教研室，江苏 徐州 221000）
  • 发布日期:2014-08-25 出版日期:2014-08-28
  • 通讯作者: 谷振勇，教授，博士研究生导师，主要从事创伤性MODS、血管生物学和呼吸肌疲劳的信号转导机制研究；E-mail：zygusz@126.com
  • 作者简介:董国凯（1981—）,男，山西侯马人，硕士，讲师，主要从事法医学教学、科研和鉴定工作；E-mail：kai2150@126.com
  • 基金资助:

    国家自然科学基金资助项目（81273341）；江苏高校优势学科建设工程资助项目（PAPD）

Nitric Oxide Mediated TNF-α, IL-1β Gene Expression in Liver Induced by Crush Injury of Rat’s Soft Tissues

DONG GUO-KAI1,2,3, ZHANG XIAO-TONG2, MA LI-QIN2, LI NA2, MA CHUN-LING2, CONG BIN2, GU ZHEN-YONG1,2   

  1. （1. Department of Forensic Medicine, Medical College of Nantong University, Nantong 226001, China; 2. Department of Forensic Medicine, Hebei Medical University, Shijiazhuang 050017, China; 3. Teaching and Research Office of Forensic Medicine, Medical College of Xuzhou, Xuzhou 221000, China）
  • Online:2014-08-25 Published:2014-08-28

摘要： 目的 探讨一氧化氮（nitric oxide，NO）在大鼠软组织挤压伤后肝组织TNF-α和IL-1β基因表达中的作用。 方法 将大鼠随机分成对照组、挤压组、挤压后氨基胍（aminoguanidine，AG）处理组、挤压后L-精氨酸（L-arginine，L-Arg）处理组，经实验处理后检测血清中ALT、AST活性和NO浓度，并利用RT-PCR技术观察肝组织中TNF-α、IL-1β基因表达情况。 结果 与对照组比较，挤压组肝组织中TNF-α、IL-1β mRNA表达明显上调（P<0.05），应用L-Arg后TNF-α、IL-1β mRNA表达进一步上调（P<0.05），而应用AG后上述指标明显下降（P<0.05）；与对照组比较，挤压组血清ALT、AST活性以及NO浓度明显增加（P<0.05），应用L-Arg后血清ALT、AST活性以及NO浓度进一步明显增高（P<0.05），应用AG后上述指标则明显降低（P<0.05）。 结论 大鼠软组织挤压伤诱导NO生成增多，内源性NO介导肝组织TNF-α和IL-1β基因表达上调。

关键词: 法医病理学, 挤压伤, 一氧化氮, 肿瘤坏死因子&alpha, 白细胞介素1&beta, 大鼠

Abstract: Objective To explore the effect of nitric oxide （NO） on the gene expression of hepatic TNF-α and IL-1β by crush injury of rat’s soft tissues. Methods Rats were randomly divided into sham group, crush group, crush+aminoguanidine （AG） group, and crush+L-arginine （L-Arg） group. Activities of ALT and AST as well as NO level in serum were measured. Gene expressions of TNF-α and IL-1β were detected with RT-PCR. Results Obvious increase in TNF-α and IL-1β mRNA expression was detected in the crush group compared with the sham group （P<0.05）. After pretreated L-Arg, expressions of TNF-α and IL-1β mRNA were markedly increased （P<0.05）. After pretreated AG, those indices obviously decreased （P<0.05）. Activities of ALT and AST enhanced and NO level increased in the crush group compared with the sham group （P<0.05）. Pretreatment with L-Arg or AG led to substantial increased or reduced activities of ALT and AST as well as NO levels, respectively. Conclusion Endogenous NO mediated TNF-α, IL-1β mRNA up expression in liver induced by increased production of NO after crush injury of rat’s soft tissues.

Key words: forensic pathology, crushing injury, nitric oxide, tumor necrosis factor-α, interleukin-1β, rats

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