法医学杂志

法医学杂志 ›› 2008, Vol. 0 ›› Issue (1): 32-33+8.

• 论文 • 上一篇    下一篇

免疫组织化学检测MMP-11鉴定月经血

姚亚楠;陆惠玲;陈森;郑晶;章雅清;   

  1. 中山大学中山医学院法医物证学教研室,中山大学中山医学院法医物证学教研室,中山大学中山医学院法医物证学教研室,中山大学中山医学院法医物证学教研室,中山大学中山医学院法医物证学教研室 广东广州510080 中山大学附属第一医院,广东广州510080,广东广州510080,广东广州510080,广东广州510080,广东广州510080
  • 发布日期:2008-02-25 出版日期:2008-02-28

Detection of MMP-11 from Menstrual Blood Using Immunohistochemistry

YAO YA-NAN1,2, LU HUI-LING2, CHEN SEN2, ZHENG JING2, ZHANG YA-QING2 (1.DEPARTMENT OF FORENSIC BIOLOGY, ZHONGSHAN SCHOOL OF MEDICINE, SUN YAT-SEN UNIVERSITY, GUANGZHOU 510080,CHINA;2.THE FIRST AFFILIATED HOSPITAL OF SUN YAT-SEN UNIVERSITY,GUANGZHOU,510080,   

  • Online:2008-02-25 Published:2008-02-28

摘要： 目的建立用自制兔抗人基质金属蛋白酶-11(matrix metalloproteinase-11,MMP-11)多克隆抗体鉴定月经血的免疫组化方法,探讨MMP-11蛋白酶的细胞定位。方法运用链霉卵白素-碱性磷酸酶(SAP)免疫组化法检测人月经血及外周静脉血涂片、阴道液涂片、子宫内膜石蜡切片和陈旧月经血痕涂片中的MMP-11。结果MMP-11存在于子宫内膜上皮细胞和间质细胞内;外周静脉血和阴道液涂片未见有MMP-11染色;陈旧月经血痕涂片罕见完整子宫内膜细胞,但能检测到微弱特异信号。结论用自制抗MMP-11多克隆抗体采用免疫组化技术能有效地检测MMP-11,区分月经血和静脉血。

关键词: 免疫组织化学, 多克隆抗体, MMP-11, 月经血

Abstract: Objective To prove the feasibility of detecting menstrual blood as well as its cellular localization with rabbit-anti-human matrix metalloproteinase-11(MMP-11) polyclonal antibody. Methods MMP-11 in menstrual blood, peripheral blood, vaginal liquid, aged menstrual bloodstain, and endometrium sections were assayed with SAP immunohistochemistry. Results MMP-11 was found only in menstrual samples within stroma and epithelium cells. Conclusion MMP-11 polyclonal antibody may be applied in the distinction between menstrual blood and venous blood.

Key words: immunohistochemistry, polyclonal antibodies, MMP-11, menstrual blood