法医学杂志

法医学杂志 ›› 2012, Vol. 28 ›› Issue (2): 109-111.DOI: 10.3969/j.issn.1004-5619.2012.02.008

• 论著 • 上一篇    下一篇

增强化学发光法检测基质金属蛋白酶-11鉴定月经血

章雅清1，2，陆惠玲2，姚亚楠3   

  1. （1． 海南省血液中心法医物证司法鉴定所，海南 海口 570311； 2． 中山大学中山医学院法医物证教研室，广东 广州 510080； 3. 中山大学附属第一医院，广东 广州 510080）
  • 发布日期:2012-04-25 出版日期:2012-04-28
  • 通讯作者: 通信作者：陆惠玲，女，教授，主要从事法医物证学研究；E-mail：luhl@mail.sysu.edu.cn
  • 作者简介:章雅清（1979—）女，山西人，硕士研究生，主要从事法医物证学研究；E-mail：fywzzyq2005@yahoo.com.cn
  • 基金资助:

    国家自然科学基金资助项目（30471933）

Detection of Matrix Metalloproteinase-11 in Menstrual Blood by Enhanced Chemiluminescence Method

ZHANG YA-QING1,2, LU HUI-LING2, YAO YA-NAN3   

  1. （1. Forensic Judical Appraisal Institute, Blood Center of Hainan Province, Haikou 570311, China; 2. Department of Forensic Biology, Zhongshan Medical College, Sun Yat-sen University, Guangzhou 510080, China; 3. The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China）
  • Online:2012-04-25 Published:2012-04-28

被引次数

2

摘要： 目的 探讨增强化学发光法检测人基质金属蛋白酶-11（matrix metalloproteinase-11，MMP-11）鉴定月经血的法医学应用价值。 方法 运用增强化学发光法检测月经血痕、阴道拭子、外周血痕、唾液斑、尿液斑和精液斑中的MMP-11，考察MMP-11的组织特异性、稳定性及检测方法的灵敏度。 结果 月经血痕中MMP-11蛋白的检测阳性率为89.47%，阴道拭子、外周血痕、唾液斑、尿液斑和精液斑中均未检出MMP-11。当上样量为25 μL时，蛋白质量浓度为1.329 μg/μL，可检出MMP-11。4 ℃放置20个月的MMP-11阳性的月经血痕，检测阳性率为89.58%。 结论 用增强化学发光法检测MMP-11蛋白的方法灵敏度高、特异性好，能用于月经血与外周血、阴道液等常见斑痕的鉴别。

关键词: 法医遗传学, 化学发光测定法, 基质金属蛋白酶-11, 月经血

Abstract: Objective To explore the forensic application value of detection of matrix metalloproteinase-11（MMP-11） in menstrual blood by enhanced chemiluminescence method. Methods Menstrual blood, vaginal swab, peripheral blood, saliva stain, urine stain and semen stain were collected to detect whether or not there were MMP-11 using enhanced chemiluminescence method. The specificity and reliability of the MMP-11 assay along with its sensitivity were evaluated. Results The positive detection rate of MMP-11 in menstrual blood was 89.47%, whereas no MMP-11 was found in vaginal swab, peripheral blood, saliva stain, urine stain and semen stain. When 25 μL sample was added, the mass concentration of protein was 1.329 μg/μL, then MMP-11 could be detected. A positive detection rate of 89.58% was observed in MMP-11 positive menstrual blood samples after stored at 4 ℃ for 20 months. Conclusion Enhanced chemiluminescence method is sensitive and specific for detecting MMP-11, and can be applied to distinguish menstrual blood from common stain such as peripheral blood, vaginal fluid.

Key words: forensic genetics, luminescent measurements, matrix metalloproteinase-11, menstrual blood

中图分类号: