法医学杂志

法医学杂志 ›› 2012, Vol. 28 ›› Issue (5): 359-361.DOI: 10.3969/j.issn.1004-5619.2012.05.009

• 技术与应用 • 上一篇    下一篇

外周血和月经血鉴别的新方法

王颖希1，朱晓君2，焦章平2，路志勇2，王伟妮1，刘雅诚2，唐  晖2   

  1. （1． 山西医科大学法医学院，山西 太原 030001； 2． 北京市公安局刑事侦查总队，北京 100192）
  • 发布日期:2012-10-25 出版日期:2012-10-28
  • 通讯作者: 唐晖，女，北京人，主任法医师，博士，主要从事法医物证学研究；E-mail：huihuitangno1@163.com
  • 作者简介:王颖希（1986—），女，山西太原人，硕士研究生，主要从事法医物证学研究；E-mail：wangyingxi4722@163.com
  • 基金资助:

    北京市科技计划课题（Z111100056811024）

A New Method of Identifying the Peripheral Blood and the Menstrual Blood

WANG YING-XI1, ZHU XIAO-JUN2, JIAO ZHANG-PING2, LU ZHI-YONG2, WANG WEI-NI1, LIU YA-CHENG2, TANG HUI2   

  1. （1. School of Forensic Medicine, Shanxi Medical University, Taiyuan 030001, China; 2. Criminal Investigation Agency, Beijing Public Security Bureau, Beijing 100192, China）
  • Online:2012-10-25 Published:2012-10-28

被引次数

6

摘要： 目的 从基因表达的组织特异性入手，寻找外周血和月经血的特异性指标，并建立对月经血和外周血进行鉴别的新方法。 方法 从检材中提取RNA后进行RT-PCR，通过电泳分离检测特异性的产物。 结果 外周血特异性的标志物膜收缩蛋白β（beta-spectrin，SPTB）和管家基因18S核糖体RNA（18S rRNA）在外周血及月经血中均表达；月经血特异性的标志物基质金属蛋白酶7（matrix metalloproteinase 7，MMP7）和阴道分泌物特异性的标志物人β防御素1（human beta defensin 1，HBD1）只在月经血中表达。 结论 外周血和月经血的基因表达存在差异，利用荧光标记结合RT-PCR检测特异性mRNA标志物的方法可以区分两者。

关键词: 法医遗传学, 月经血, 外周血, 鉴别

Abstract: Objective To explore the tissue-specific gene expressions of the peripheral blood and the menstrual blood, and to search some specific factors to establish an effective method for identifying the peripheral blood and the menstrual blood. Methods The specific products of the peripheral blood and the menstrual blood were detected by RT-PCR and separated by electrophoretic technology. Results Beta-spectrin （SPTB） as one specific marker of peripheral blood and 18S rRNA as a kind of the housekeeping gene were expressed in both the peripheral blood and the menstrual blood. However, matrix metalloproteinase 7 （MMP7） as one specific marker of menstrual blood and human beta defensin 1 （HBD1） as one specific marker of vaginal discharge were only found in the menstrual blood. Conclusion There are differences of specific gene expressions between the peripheral blood and the menstrual blood. They could be accurately distinguished from each other by using the combination of fluorescence technology and RT-PCR to detect the specific identification of mRNA.

Key words: forensic genetics, menstrual blood, peripheral blood, identification

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