法医学杂志

法医学杂志 ›› 2008, Vol. 0 ›› Issue (2): 129-130+.

• 论文 • 上一篇    下一篇

荧光定量PCR技术验证Sinofiler试剂盒的适宜模板量

李成涛;郭宏;林源;柳燕;阙庭志;李莉;   

  1. 司法部司法鉴定科学技术研究所上海市法医学重点实验室;苏州大学基础医学院;
  • 发布日期:2008-04-25 出版日期:2008-04-28

Analysis of Appropriate Amount of Template DNA for Sinofiler Kit by Real Time Quantitative PCR Technique

LI CHENG-TAO1,2,GUO HONG3,LIN YUAN1,LIU YAN1,QUE TING-ZHI1,LI LI1(1.SHANGHAI KEY LABORATORY OF FORENSIC MEDICINE,INSTITUTE OF FORENSIC SCIENCE,MINISTRY OF JUSTICE,P.R.CHINA,SHANGHAI 200063,CHINA;2.SCHOOL OF LIFE SCIENCES,FUDAN UNIVERSITY,SHANGHAI 200433,C   

  • Online:2008-04-25 Published:2008-04-28

被引次数

21

摘要： 目的探讨Sinofiler试剂盒适合扩增的模板DNA用量。方法选取经Sinofiler试剂盒基因分型且图谱完整、扩增均衡性好的DNA样本,进行荧光定量PCR检测。结果实验结果表明,在12.5μL体系中,1.29～1.51ng的模板DNA能够获得理想的分型结果。结论应用荧光定量PCR技术检测不同试剂盒适合扩增的模板DNA用量是一种准确、有效的方法。

关键词: 法医生物学, 荧光定量PCR技术, 定量, Sinofiler试剂盒

Abstract: Objective To explore the appropriate amount of template DNA for Sinofiler Kit.Methods The DNA samples with ideally genotyped results by Sinofiler Kit were detected by real-time quantitative PCR assay.Results It was shown that 1.29-1.51 ng of template DNA in 12.5 μL reaction volume was optimal for STR genotyping with Sinofiler Kit.Conclusion Real time quantitative PCR is an accurate and necessary technique for detection of appropriate amount of template DNA for different kits.

Key words: forensic biology, fluorescent quantitative PCR, quantification, Sinofiler kit