法医学杂志

法医学杂志 ›› 2008, Vol. 0 ›› Issue (6): 423-424+.

• 论文 • 上一篇    下一篇

放置时间对烟蒂上DNA含量及STR分型的影响

张爱平;陈晓晖;刘长晖;陈丽伟;李健伟;刘超;   

  1. 中山大学中山医学院法医学系;广州市刑事科学技术研究所;
  • 发布日期:2008-12-25 出版日期:2008-12-28

The Effect on DNA Quantification and STR Typing from Cigarette Butts Collected at Various Time Points

ZHANG AI-PING1, CHEN XIAO-HUI2, LIU CHANG-HUI2, CHEN LI-WEI1, LI JIAN-WEI1, LIU CHAO1,2 (1. DEPARTMENT OF FORENSIC MEDICINE, ZHONGSHAN MEDICAL COLLEGE, SUN YAT-SEN UNIVERSITY, GUANGZHOU 510080, CHINA; 2. GUANGZHOU INSTITUTE OF CRIMINAL SCIENCE AND TECHNOL   

  • Online:2008-12-25 Published:2008-12-28

被引次数

2

摘要： 目的探讨不同放置时间对烟蒂上DNA含量及STR分型的影响。方法以10名志愿者的40枚"红双喜"烟蒂为检材(每人4枚),用chelex-100法分别在第1、4、7和10周时提取烟蒂样本外层烟纸和海绵上的DNA,用Quantifiler人类DNA定量试剂盒进行DNA定量,并同时用AmpFL STR Profiler和IdentifilerTM试剂盒(AB公司)进行PCR扩增及STR分型。结果放置第1、4、7和10周的烟蒂烟纸DNA质量浓度范围分别为0.104～2.52、0.110～2.41、0.0960～2.32和0.0850～2.28ng/μL,16个基因座检出率分别为100%、90%、75%和62.5%。海绵DNA质量浓度范围分别为0.0180～2.40、0.0171～2.25、0.0165～2.15和0.0160～2.15ng/μL,16个基因座检出率分别为97.5%、82.5%、50%和12.5%。结论放置时间对烟纸和海绵上的DNA含量影响不大,对STR分型影响显著,STR分型受海绵的影响比烟纸明显。放置时间越久,检出率越低。

关键词: 法医遗传学, 口腔黏膜, 细胞提取物, 烟蒂, DNA定量, STR分型

Abstract: Objective To explore the effect on DNA quantification and STR typing from cigarette butts collected at different time points. Methods Forty "Hongshuangxi" brand cigarette butts smoked by ten different individuals(4 cigarettes per individual) were collected. DNA was extracted from the outer layer and the sponge of the cigarette butts using chelex-100 extraction kit, as well as STR typing and DNA quantitation were simultaneously performed in 1, 4, 7 and 10 weeks, respectively. Results The DNA quantities extracted from the outer layer at the 1st, 4th, 7th and 10th week were 0.104-2.52, 0.110-2.41, 0.096 02.32 and 0.085 0-2.28 ng/μL, while the detection rates for 16 loci by STR typing were 100%, 90%, 75% and 62.5%, respectively. The DNA quantities extracted from the sponge were 0.018 0-2.40, 0.017 1-2.25, 0.016 5-2.15 and 0.016 0-2.15 ng/μL, while the detection rates for 16 loci by STR typing were 97.5%, 82.5%, 50% and 12.5%, respectively. Conclusion There is little difference in DNA quantity between the outer layer and the sponge of butts during 10 weeks, but there is an obvious effect on STR typing with prolonged extracting time. There is a much more effect on the sponge than on the outer layer, and the longer the standing time is, the lower the detection rate is.

Key words: forensic genetics, mouth mucosa, cell extracts, cigarette butt, DNA quantification, STR typing